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The combined use of photobiomodulation and curcumin-loaded iron oxide nanoparticles significantly improved wound healing in diabetic rats compared to either treatment alone

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Abstract

This experimental study examined the effects of curcumin-loaded iron oxide nanoparticles (CUR), photobiomodulation (PBM), and CUR + PBM treatments on mast cells (MC)s numbers and degranulation, inflammatory cells (macrophages, neutrophils), and wound strength in the last step of the diabetic wound repair process (maturation phase) in a rat model of type one diabetes mellitus (T1DM). T1DM was induced in 24 rats, and 1 month later, an excisional wound was created on each rat’s back skin. The rats were then distributed into four groups: (1) untreated diabetic control group (UDCG); (2) rats treated with CUR (CUR); (3) rats exposed to PBM (890 nm, 80 Hz, 0.2 J/cm2) (PBM); (4) rats treated with CUR plus PBM (CUR + PBM). Fifteen days after surgery, skin tissue samples were taken for biomechanical and stereological evaluations. The biomechanical factor of maximum force was observed to be considerably improved in the CUR + PBM (p = 0.000), PBM (p = 0.014), and CUR (p = 0.003) groups compared to the UDCG. CUR + PBM, PBM, and CUR groups had significantly decreased total numbers of MC compared with the UDCG (all, p = 0.001). The results were significantly better in the CUR + PBM (p = 0.000) and PBM (p = 0.003) groups than in the CUR group. Inflammatory cell counts were significantly lower in the CUR + PBM, PBM, and CUR groups than in the UDCG (all, p = 0.0001). In all evaluating methods, the usage of CUR + PBM produced better results than the use of CUR or PBM alone (almost all tests, p = 0.0001). CUR + PBM, PBM, and CUR significantly improved the repair of diabetic skin wounds in type 1 DM rats through significant decreases of MC number, degranulation, and inflammatory cells as well as a noteworthy improvement in wound strength. The impact of CUR + PBM was superior to that of either PBM or CUR alone. It is suggested that CUR + PBM could be used as a MC stabilizer for the effective treatment of some related human diseases.

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Funding

The present article is financially supported by “Research Department of the School of Medicine” at Shahid Beheshti University of Medical Sciences, Tehran, Iran (Grant No.:30491), (IR.SBMU.MSP.REC.1400. 675).

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Authors and Affiliations

  1. School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Ahmadreza Ardeshirzadeh

  2. Department of Biology and Anatomical Sciences, Shahid Beheshti University of Medical Sciences (SBMU), Tehran, Iran

    Houssein Ahmadi, Hamidreza Omidi, Abdollah Amini & Mohammad Bayat

  3. Department of Anatomy, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran

    Mansooreh Mirzaei

  4. Department of Anatomy, Faculty of Medicine, Medical Sciences, Islamic Azad University, Tehran, Iran

    Atarodalsadat Mostafavinia

  5. Illinois Institute of Technology, Chicago, IL, USA

    Sahar Bayat

  6. Department of Biology and Anatomical Sciences, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran

    Mohammadjavad Fridoni

  7. Price Institute of Surgical Research, University of Louisville and Noveratech LLC, Louisville, KY, USA

    Sufan Chien & Mohammad Bayat

Authors
  1. Ahmadreza Ardeshirzadeh
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Contributions

MB wrote the manuscript. AA, HA, MM, HO, and SB performed the experiments. AA and MF added their comments. SC reviewed and edited the manuscript. AM performed the statistical methods. All the authors confirmed the manuscript.

Corresponding authors

Correspondence to Sufan Chien or Mohammad Bayat.

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All procedures were approved by the IRB of Shahid Beheshti University of Medical Sciences (IR.SBMU.MSP.REC.1400. 675).

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Ardeshirzadeh, A., Ahmadi, H., Mirzaei, M. et al. The combined use of photobiomodulation and curcumin-loaded iron oxide nanoparticles significantly improved wound healing in diabetic rats compared to either treatment alone. Lasers Med Sci 37, 3601–3611 (2022). https://doi.org/10.1007/s10103-022-03639-4

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