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Monte Carlo simulations of photodynamic therapy in human blood model

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Abstract

This study aims to simulate a therapeutic plan for a normal human blood model under various patho-physiological conditions, such as the development of leukemia/blood diseases, by means of Monte Carlo multilayered simulation. The photosensitizing compound selectively accumulates in the target cells. A superficial treatment of a blood sample was performed at different ratios of oxygen saturation (\({f}_{oxy} = 50{\%}, 53{\%}, 55{\%}, 60{\%}, 65{\%},{ and }70{\%}\)) under the concentration (\({C}_{{MC}540}\) = 30 µM) effect of merocyanine 540 (MC540) in the blood irradiation. This was done under the application of visible light of wavelength ~ \(580{ nm}\) at an exposure time ~ 60 s. The dose of photodynamic therapy (PDT) was evaluated for the biological damage, leading to necrosis and blood damage during the treatment. In addition, the effect of PDT treatment response in the blood is related to hemoglobin oxygen saturation, resulting in an excellent relationship between the changes caused by the treatment in the blood at a peculiar oxygen saturation rate (for the highest response: \({f}_{oxy}=\) 50%) and a light dose (LD) of 3.83 \({{Jcm}}^{-2}\) above the minimal toxicity of normal tissues. The photodynamic dose is related to the depth of necrosis and the time of treatment for the achievement of the LD delivery at the PDT of blood.

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taken from previous work [13])

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source powers (P = 10, 30, 50, 70, and 100 (mW)) with CMC540 = 30 μM

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Authors and Affiliations

  1. Department of Physics and Astronomy, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia

    R. S. Alanazi & A. Laref

  2. Department of Biomedical Technology, College of Applied Medical Sciences, King Saud University, P.O. Box 10219, Riyadh, Saudi Arabia

    R. S. Alanazi

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Alanazi, R.S., Laref, A. Monte Carlo simulations of photodynamic therapy in human blood model. Lasers Med Sci 37, 1515–1529 (2022). https://doi.org/10.1007/s10103-021-03383-1

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