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Laser treatment contributes to maintain membrane integrity in stem cells from human exfoliated deciduous teeth (shed) under nutritional deficit

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Abstract

This study aimed to analyze the effects of laser irradiation on the membrane integrity and viability of stem cells from human exfoliated deciduous teeth (SHED) that were kept in serum starvation. Nutritional deficit was used to mimic the cellular stress conditions of SHED isolation for regenerative dental approaches, where laser therapy could be beneficial. SHED were cultured under serum starvation (MEMα + 1%FBS) for 1 or 24 h pre-irradiation (protocols A and B, respectively). Then, cells received low-level laser therapy (LLLT; 660 nm) at 2.5 J/cm2 (0.10 W; groups I and V), 5.0 J/cm2 (0.20 W; groups II and VI), 7.5 J/cm2 (0.30 W; groups III and VII), or remained non-irradiated (groups IV and VIII). During irradiation, cells were maintained in 1% FBS (groups I–IV) or 10% FBS (normal culture conditions; groups V–VIII). Membrane integrity was evaluated by quantifying lactate dehydrogenase (LDH) release (immediately after irradiation), and cell viability was assessed by the MTT assay (24, 48, and 72 h post-irradiation). Serum starvation did not alter LDH release by non-irradiated SHED, while LDH release decreased significantly in groups irradiated in 1% FBS (I and III), but not in groups irradiated in 10% FBS (V–VII), regardless the pre-irradiation conditions (protocols A/B). Cell viability was significantly higher 24 h after irradiation, in most protocol A groups. In contrast, cell viability remained mostly unaltered in protocol B groups. LLLT contributed to maintain membrane integrity in SHED subjected to nutritional deficit before and during irradiation with 0.10 or 0.30 W. Short serum starvation before irradiation improved SHED viability at 24 h post-irradiation.

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Funding

This study was financially supported by Minas Gerais Research Foundation (FAPEMIG, Brazil, no. APQ-04004-16) and Coordination for the Improvement of Higher Education Personnel (CAPES, Brazil, no. 88881.068437/2014–1).

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Authors and Affiliations

  1. Department of Clinics and Surgery, School of Dentistry, Federal University of Alfenas (UNIFAL-MG), Rua Gabriel Monteiro da Silva, 700., Alfenas, Minas Gerais, 37130-001, Brazil

    Paula Corrêa Silveira da Silva, Nelson Pereira Marques, Marcella Tassi Farina, Nádia Carolina Teixeira Marques & Vivien Thiemy Sakai

  2. Department of Pediatric Dentistry, Orthodontics and Public Health, Bauru School of Dentistry, University of São Paulo (USP), Bauru, São Paulo, Brazil

    Thais Marchini Oliveira

  3. Department of Pediatric Dentistry and Public Health, School of Dentistry, São Paulo State University (UNESP), Araçatuba, Brazil

    Cristiane Duque

  4. Department of Pediatric Dentistry, School of Dentistry, José do Rosário Vellano University (UNIFENAS), Alfenas, Minas Gerais, Brazil

    Nádia Carolina Teixeira Marques

Authors
  1. Paula Corrêa Silveira da Silva
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  2. Nelson Pereira Marques
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  3. Marcella Tassi Farina
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  4. Thais Marchini Oliveira
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  5. Cristiane Duque
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  6. Nádia Carolina Teixeira Marques
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  7. Vivien Thiemy Sakai
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Corresponding author

Correspondence to Vivien Thiemy Sakai.

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This study was conducted after approval by the Ethics Committee of Institute of Science and Technology, São Paulo State University (under protocol 46420).

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The authors declare that they have no conflict of interest.

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da Silva, P.C.S., Marques, N.P., Farina, M.T. et al. Laser treatment contributes to maintain membrane integrity in stem cells from human exfoliated deciduous teeth (shed) under nutritional deficit. Lasers Med Sci 34, 15–21 (2019). https://doi.org/10.1007/s10103-018-2574-x

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  • DOI: https://doi.org/10.1007/s10103-018-2574-x

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