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The influence of low-intensity He-Ne laser on the wound healing in diabetic rats

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Abstract

The low-level laser irradiation at certain wavelengths is reported to facilitate the healing process of diabetic wounds. Thus, this study carried out to look for the suitable laser parameters that could speed up the healing process. Fourteen healthy male and female rats were used in which a circular wound with a diameter of 2.5 ± 0.2 cm was created on the dorsum in each rat after injected them with alloxan to induced diabetic. They have been divided into two groups: control group (N = 7) and study group (N = 7) to conduct the study. He-Ne laser with a wavelength of 632.8 nm at power density of 4.0 mW/cm2 was used to irradiate the study group for five times a week until the wound healed (closed) completely, while the control group was kept untreated. The results showed that the laser-treated group healed (wounds were totally closed) faster compared to the control group. In numbers, the laser-treated group healed on average at the 21st day (0.0 ± 0.0 cm) (P ≤ 0.005), whereas the control group healed after 40 days or even 60 days in some cases (sample no. 2). This confirms that laser promotes the tissue repair process of diabetic wounds and reduces the healing period to the half.

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Acknowledgements

The authors would like to thank Dr. Shama Yunes, Department Biochemistry, and Dr. Abd Alfatah Mohamed, Department of Biomedical Physics, Alneelain University, for their technical support. The authors would like to thank Dr. Atif Idrees, Department of Biology, Alneelain University, for his kind assistance in data analysis.  Thanks is extended to Dr. Tahany Osman, Department of Biochemistry, Bahry University, for her contribution in identifying the doses of Alloxan and Mr. Basheer Ismail, Faculty of Pharmacy, Omdurman Islamic University, for his assistance in injecting animals and the samples collection.

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Authors and Affiliations

  1. Department of Laser, Alneelain University, Khartoum, Sudan

    Maha Eissa

  2. Department of Biomedical Physics, Alneelain University, Khartoum, Sudan

    Wasil H. M. Salih

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  1. Maha Eissa
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  2. Wasil H. M. Salih
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Correspondence to Wasil H. M. Salih.

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All procedures performed in this study were according to the local ethical committee of Alneelian University, the Sudan, and were in accordance to the International Guiding Principles for Research Involving Animals and Human Beings.

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Eissa, M., Salih, W.H.M. The influence of low-intensity He-Ne laser on the wound healing in diabetic rats. Lasers Med Sci 32, 1261–1267 (2017). https://doi.org/10.1007/s10103-017-2230-x

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  • DOI: https://doi.org/10.1007/s10103-017-2230-x

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