Abstract
In this study, we aimed to investigate the wavelength-dependent effects of hair growth on the shaven backs of Sprague–Dawley rats using laser diodes with wavelengths of 632, 670, 785, and 830 nm. Each wavelength was selected by choosing four peak wavelengths from an action spectrum in the range 580 to 860 nm. The laser treatment was performed on alternating days over a 2-week period. The energy density was set to 1.27 J/cm2 for the first four treatments and 1.91 J/cm2 for the last four treatments. At the end of the experiment, both photographic and histological examinations were performed to evaluate the effect of laser wavelength on hair growth. Overall, the results indicated that low-level laser therapy (LLLT) with a 830-nm wavelength resulted in greater stimulation of hair growth than the other wavelengths examined and 785 nm also showed a significant effect on hair growth.
Similar content being viewed by others
References
Mester E, Szende B, Gartner P (1968) The effect of laser beams on the growth of hair in mice. Radiobiol Radiother (Berl) 9(5):621–626
Satino JL, Markou M (2003) Hair regrowth and increased hair tensile strength using the HairMax LaserComb for low-level laser therapy. Int J Cosmet Surg Aesthet Dermatol 5(2):113–117
Shukla S, Sahu K, Verma Y, Rao KD, Dube A, Gupta PK (2010) Effect of helium-neon laser irradiation on hair follicle growth cycle of Swiss albino mice. Skin Pharmacol Physiol 23(2):79–85
Lanzafame RJ, Blanche RR, Bodian AB, Chiacchierini RP, Fernandez-Obregon A, Kazmirek ER (2013) The growth of human scalp hair mediated by visible red light laser and LED sources in males. Lasers Surg Med 45:487–495
Chung H, Dai T, Sharma SK, Huang YY, Carroll JD, Hamblin MR (2012) The nuts and bolts of low-level laser (light) therapy. Ann Biomed Eng 40(2):516–533
Leavitt M, Charles G, Heyman E, Michaels D (2009) HairMax LaserComb laser phototherapy device in the treatment of male androgenetic alopecia: a randomized, double-blind, sham device-controlled, multicentre trial. Clin Drug Investig 29(5):283–292
Avci P, Gupta GK, Clark J, Wikonkal N, Hamblin MR (2014) Low‐level laser (light) therapy (LLLT) for treatment of hair loss. Lasers Surg Med 46(2):144–151
Hamblin MR, Demidova TN (2006) Mechanisms of low level light therapy. Proc SPIE 6140:614001–614012
Rangwala S, Rashid RM (2012) Alopecia: a review of laser and light therapies. Dermatol Online J 18(2):3
King LE Jr, Silva KA, Kennedy VE, Sundberg JP (2014) Lack of response to laser comb in spontaneous and graft induced alopecia areata in C3H/HeJ mice. J Investig Dermatol 134(1):264–266
Wikramanayake TC, Rodriguez R, Choudhary S, Mauro LM, Nouri K, Schachner LA, Jimenez JJ (2012) Effects of the Lexington LaserComb on hair regrowth in the C3H/HeJ mouse model of alopecia areata. Lasers Med Sci 27(2):431–436
Karu TI (2010) Multiple roles of cytochrome c oxidase in mammalian cells under action of red and IR-A radiation. IUBMB Life 62(8):607–610
Hartmann KM (1983) Action spectroscopy. In: Hoppe W, Lohmann W, Marke H, Ziegler H (eds) Biophysics. Springer, Heidelberg, pp 115–144
Liu LY, Guo DS, Xin XY, Fang J (2008) Observation of a system of linear loops formed by re-growing hairs on rat skin. Anat Rec (Hoboken) 291(7):858–868
Tzung TY, Yang CY, Huang YC, Kao FJ (2009) Colorimetry provides a rapid objective measurement of de novo hair growth rate in mice. Skin Res Technol 15(4):459–463
Karu TI, Pyatibrat LV, Afanasyeva NI (2004) A novel mitochondrial signaling pathway activated by visible-to-near infrared radiation. Photochem Photobiol 80(2):366–372
Lee S, Kim TH, Youn JI (2014) Development of the theragnostic optical system for a high-intensity laser therapy (HILT). Lasers Med Sci 29(5):1585–1591
Almeida-Lopes L, Rigau J, Zângaro RA, Guidugli-Neto J, Jaeger MM (2001) Comparison of the low level laser therapy effects on cultured human gingival fibroblasts proliferation using different irradiance and same fluence. Lasers Surg Med 29(2):179–184
Evans DH, Abrahamse H (2008) Efficacy of three different laser wavelengths for in vitro wound healing. Photodermatol Photoimmunol Photomed 24(4):199–210
Alster TS, Bryan H, Williams CM (2001) Long-pulsed Nd:YAG laser-assisted hair removal in pigmented skin: a clinical and histological evaluation. Arch Dermatol 137(7):885–889
Kim TH, Lee GW, Youn JI (2014) A comparison of temperature profile depending on skin types for laser hair removal therapy. Lasers Med Sci 29(6):1829–1837
Makihara E, Masumi S (2008) Blood flow changes of a superficial temporal artery before and after low-level laser irradiation applied to the temporomandibular joint area. Nihon Hotetsu Shika Gakkai Zasshi 52(2):167–170
Makihara E, Makihara M, Masumi S, Sakamoto E (2005) Evaluation of facial thermographic changes before and after low-level laser irradiation. Photomed Laser Surg 23(2):191–195
Wikramanayake TC, Villasante AC, Mauro LM, Nouri K, Schachner LA, Perez CI, Jimenez JJ (2013) Low-level laser treatment accelerated hair regrowth in a rat model of chemotherapy-induced alopecia (CIA). Lasers Med Sci 28(3):701–706
Acknowledgments
This work was supported by research grants from the Catholic University of Daegu in 2014.
Compliance with Ethical Standards
All procedures performed in this study involving animals were in accordance with the ethical standards of the Animal Care and Use Committee of the Catholic University of Daegu (approval no. CUD-2014-33).
Conflict of interest
The authors declare that they have no competing interests.
Ethical approval
All procedures performed in studies involving animals were in accordance with the ethical standards of the institution at which the studies were conducted (CUD-2014-033).
Author information
Authors and Affiliations
Corresponding author
Electronic supplementary material
Below is the link to the electronic supplementary material.
Online Resource 1
(PDF 221 kb)
Rights and permissions
About this article
Cite this article
Kim, TH., Kim, NJ. & Youn, JI. Evaluation of wavelength-dependent hair growth effects on low-level laser therapy: an experimental animal study. Lasers Med Sci 30, 1703–1709 (2015). https://doi.org/10.1007/s10103-015-1775-9
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10103-015-1775-9