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Dissemination of blaOXA-370 is mediated by IncX plasmids and the Tn6435 transposon

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Abstract

In Enterobacteriaceae, the blaOXA-48-like genes have been identified on plasmids in different regions of the world. The OXA-370 is a plasmid-encoded OXA-48-like enzyme reported in two distinct regions of Brazil. Recently, we demonstrate that the blaOXA-370 gene is disseminated among several Enterobacteriaceae species and clones, indicating a high potential for dissemination. In this work, we described for the first time the complete nucleotide sequence of six plasmids harboring the blaOXA-370 gene. Complete DNA sequencing using the Illumina platform and annotation of the plasmids showed that they belonged to incompatibility groups IncX and had in average 70 kbp. The blaOXA-370 gene is located in a composite transposon containing four genes encoding transposases, named Tn6435. In this study, highly similar plasmids were detected in different Enterobacteriaceae genera.

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Funding

This work was supported by the Fundo de Incentivo à Pesquisa e Eventos do Hospital de Clínicas de Porto Alegre, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, National Council for Scientific and Technological Development (CNPq), Ministry of Science and Technology, Brazil, and Fleury Institute.

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Author notes

  1. Alexandre P. Zavascki and Jorge L. M. Sampaio are both senior authors and contributed equally to the work.

Authors and Affiliations

  1. Laboratório de Pesquisa em Resistência Bacteriana (LABRESIS) – Centro de Pesquisa Experimental, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil

    Cibele M. Magagnin, Franciéli P. Rozáles & Afonso L. Barth

  2. Programa de Pós-Graduação em Medicina: Ciências Médicas, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil

    Cibele M. Magagnin

  3. Microbiology Section, Fleury Medicine and Health, São Paulo, Brazil

    Cibele M. Magagnin & Jorge L. M. Sampaio

  4. School of Pharmacy, Clinical Microbiology Laboratory, University of São Paulo, São Paulo, Brazil

    Juliana C. Campos, Darlan A. da Rocha & Jorge L. M. Sampaio

  5. Santa Casa de São Paulo School of Medical Sciences, São Paulo, Brazil

    Suely C. F. Sampaio

  6. Infectious Diseases Service, Hospital Moinhos de Vento, Porto Alegre, Brazil

    Alexandre P. Zavascki

  7. Department of Internal Medicine, Medical School, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil

    Alexandre P. Zavascki

Authors
  1. Cibele M. Magagnin
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  2. Juliana C. Campos
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  3. Darlan A. da Rocha
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  4. Suely C. F. Sampaio
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  5. Franciéli P. Rozáles
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  6. Afonso L. Barth
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  7. Alexandre P. Zavascki
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  8. Jorge L. M. Sampaio
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Corresponding author

Correspondence to Cibele M. Magagnin.

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Conflict of interest

A. P. Z. has received honoraria for speaking engagements and consultancy from Merck, AstraZeneca, Pfizer, and United Pharmaceuticals. All other authors have no conflicts of interest to declare.

Ethical approval

The study was approved by the Ethical Committee of Hospital de Clínicas de Porto Alegre (14-0046).

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Magagnin, C.M., Campos, J.C., da Rocha, D.A. et al. Dissemination of blaOXA-370 is mediated by IncX plasmids and the Tn6435 transposon. Eur J Clin Microbiol Infect Dis 37, 2165–2169 (2018). https://doi.org/10.1007/s10096-018-3356-x

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  • DOI: https://doi.org/10.1007/s10096-018-3356-x

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