Abstract
Ischemic stroke (IS) is a major cause of mortality and disability. However, no reliable prognostic or diagnostic biomarker has been utilized to date. Here, we have evaluated the serum S100B concentration and miR-602 expression as potential biomarkers for IS. Fifty-two IS patients and 52 age- and sex-matched healthy volunteers were enrolled. Blood samples were collected from all patients at the time of admission, 24 and 48 h later, at the time of discharge, and 3 months later. Real-time (RT) PCR was used to measure the serum level of miR602. We also measured the serum concentration of S100B using ELISA. As compared with healthy subjects, IS patients had a higher level of serum S100B and lower serum miR-602. ROC curve analyses revealed that miR-602 (AUC = 0.8168; P < 0.0001) and S100B (AUC = 0.8699; P < 0.0001) had acceptable ability to differentiate between IS patients from healthy subjects. Furthermore, serum S100B was a reliable predictor of the survival outcome at 3 months (P = 0.021). The expression of miR-602 was significantly higher in patients with bigger NIHSS scores. The lower levels of miR-602 and higher concentration of S100B in the sera of IS patients could be associated with clinically significant diagnostic utilities. S100B could be also introduced as a reliable prognostic marker for stroke and implemented in future research.
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Acknowledgments
Data for this article was derived from a M.Sc. thesis in the field of Clinical Biochemistry written by Mina Rahmati at Gorgan School of Medicine of Golestan University of Medical Sciences, Gorgan, Iran.
Funding
The current study was financially supported by the Deputy of Research and Technology, Golestan University of Medical Sciences (Grant number: 960427092).
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MR: acquisition of data, analyses and interpretations of data, manuscript drafting, revision of the manuscript. MRAP: participation in data acquisition. HE: participation in data analysis. GAF: participation in data acquisition and manuscript drafting. MGM: participation in data acquisition and manuscript drafting. HG: participation in data acquisition. NM: study design and concept, participation in literature bibliography, data acquisition and analysis, manuscript drafting, and critical revision of the manuscript. All authors read and approved the final version of the manuscript.
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The study was approved by the Committee of Ethics, Golestan University of Medical Sciences (IR.GOUMS.REC.1395.23). All patients signed a written informed consent according to the declaration of Helsinki.
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Rahmati, M., Azarpazhooh, M.R., Ehteram, H. et al. The elevation of S100B and downregulation of circulating miR-602 in the sera of ischemic stroke (IS) patients: the emergence of novel diagnostic and prognostic markers. Neurol Sci 41, 2185–2192 (2020). https://doi.org/10.1007/s10072-020-04323-7
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DOI: https://doi.org/10.1007/s10072-020-04323-7