Skip to main content
SpringerLink
Log in

BEFS-PPARγ2 cells incubated with trans-11 C18:1 exhibit more beneficial fatty acid synthesis

  • Research Note
  • Published:
Food Science and Biotechnology Aims and scope Submit manuscript

Abstract

Responses of the PPARγ2 (an adipogenic lineage determinant) over-expressed immortalized bovine embryonic fibroblast cell line (BEFS-PPARγ2 cells) to trans-11 C18:1 (TVA) incubation were evaluated. BEFS-PPARγ2 cells were well differentiated. Stearoyl-CoA desaturase 1, an endoplasmic reticulum enzyme that can catalyze conversion of TVA into cis-9, trans-11 CLA, exhibited a significantly (p<0.05) higher mRNA expression after 4 h, compared with controls. Enzyme activity peaked, and was significantly (p<0.05) higher after 6 h, compared with controls. With extension of the incubation time, TVA absorption into cells was significantly (p<0.05) higher with significantly (p<0.05) more conversion into cis-9, trans-11 CLA, compared with shorter incubation times. TVA incubated cells exhibited significantly (p<0.05) higher levels of arachidonic, eicosapentaenoic, and docosahexaenoic acids than controls. BEFS-PPARγ2 cells can be used as a model system and TVA incubation is a good approach for research into cis-9, trans-11 CLA-fortified beef production.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Wang T, Lee HG. Advances in research on cis-9, trans-11 conjugated linoleic acid: A major functional conjugated linoleic acid isomer. Crit. Rev. Food Sci. 55: 720–731 (2015)

    Article  CAS  Google Scholar 

  2. Dhiman TR, Nam SH, Ure AL. Factors affecting conjugated linoleic acid content in milk and meat. Crit. Rev. Food Sci. 45: 463–482 (2005)

    Article  CAS  Google Scholar 

  3. Rodríguez-Alcalá LM, Fontecha J. Hot topic: Fatty acid and conjugated linoleic acid (CLA) isomer composition of commercial CLA-fortified dairy products: Evaluation after processing and storage. J. Dairy Sci. 90: 2083–2090 (2007)

    Article  Google Scholar 

  4. Wang T, Oh JJ, Lim JN, Hong JE, Kim JH, Kim JH, Kang HS, Choi YJ, Lee HG. Effects of lactation stage and individual performance on milk cis-9, trans-11 conjugated linoleic acids content in dairy cows. Asian Austral. J. Anim. 26: 189–194 (2013)

    Article  CAS  Google Scholar 

  5. Wang T, Lim JN, Bok JD, Kim JH, Kang SK, Lee SB, Hwang JH, Lee KH, Kang HS, Choi YJ, Kim EJ, Lee HG. Association of protein expression in isolated milk epithelial cells and cis-9, trans-11 conjugated linoleic acid proportions in milk from dairy cows. J. Sci. Food Agr. 94: 1835–1843 (2014)

    Article  CAS  Google Scholar 

  6. Campbell W, Drake MA, Larick DK. The impact of fortification with conjugated linoleic acid (CLA) on the quality of fluid milk. J. Dairy Sci. 86: 43–51 (2003)

    Article  CAS  Google Scholar 

  7. Lock AL, Bauman DE. Modifying milk fat composition of dairy cows to enhance fatty acids beneficial to human health. Lipids 39: 1197–1206 (2004)

    Article  CAS  Google Scholar 

  8. Yin JL, Jin X, Beck SU, Kang DH, Hong Z, Li ZH, Jin YC, Zhang QK, Choi YJ, Kim SC, Kim HG. In vitro myogenic and adipogenic differentiation model of genetically engineered bovine embryonic fibroblast cell lines. Biotechnol. Lett. 32: 195–202 (2010)

    Article  CAS  Google Scholar 

  9. Folch J, Lees M, Sloane Stanley GH. A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226: 497–509 (1957)

    CAS  Google Scholar 

  10. Wang T, Lim JN, Choi SH, Kang HS, Lee HG. Comparative studies on derivatization methods of single or mixed fatty acids. Food Sci. Biotechnol. 22: 1573–1579 (2013)

    Article  CAS  Google Scholar 

  11. Kay JK, Mackle TR, Auldist MJ, Thomson NA, Bauman DE. Endogenous synthesis of cis-9, trans-11 conjugated linoleic acid in dairy cows fed fresh pasture. J. Dairy Sci. 87: 369–378 (2004)

    Article  CAS  Google Scholar 

  12. Mosley EE, Shafii Dagger B, Moate PJ, McGuire MA. cis-9, trans-11 conjugated linoleic acid is synthesized directly from vaccenic acid in lactating dairy cattle. J. Nutr. 136: 570–575 (2006)

    CAS  Google Scholar 

  13. Bell JA, Griinari JM, Kennelly JJ. Effect of safflower oil, flaxseed oil, monensin, and vitamin E on concentration of conjugated linoleic acid in bovine milk fat. J. Dairy Sci. 89: 733–748 (2006)

    Article  CAS  Google Scholar 

  14. Rapoport SI. Arachidonic acid and the brain. J. Nutr. 138: 2515–2520 (2008)

    CAS  Google Scholar 

  15. Ohnishi H, Saito Y. Eicosapentaenoic acid (EPA) reduces cardiovascular events: Relationship with the EPA/arachidonic acid ratio. J. Atheroscler. Thromb. 20: 861–877 (2013)

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China

    Tao Wang, LiFang Wu, GuiXin Qin, YuJie Lou, ZeWei Sun & Jian Yang

  2. Department of Animal Science and Technology, College of Animal Bioscience and Technology, Konkuk University, Seoul, 143-701, Korea

    HongGu Lee

  3. Animal Husbandry Station of Jilin Province, Changchun, 130118, China

    Wei Guo

Authors
  1. Tao Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. HongGu Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. LiFang Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. GuiXin Qin
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. YuJie Lou
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. ZeWei Sun
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Wei Guo
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Jian Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Tao Wang.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Wang, T., Lee, H., Wu, L. et al. BEFS-PPARγ2 cells incubated with trans-11 C18:1 exhibit more beneficial fatty acid synthesis. Food Sci Biotechnol 24, 1893–1896 (2015). https://doi.org/10.1007/s10068-015-0248-z

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10068-015-0248-z

Keywords

Search

Navigation