Abstract
WE (water extraction at 100°C for 24 h), EE (70% ethanol extraction at 80°C for 24 h), and AWE (alkaline reduced water extraction at 100°C for 24 h) were performed. Nitric oxide production from macrophages of AWE extracts at 1.0 mg/mL was decreased to 3.1 μmol. Secretion of PGE2 from human fibroblasts was also reduced to 496.47 pg/mL at 1.0 mg/mL of AWE extracts with UV irradiation. Anti-skin inflammatory activities were enhanced by extraction of 8.894 mg/g more baicalein, the major bioactive component in Scutellaria baicalensis, using alkaline reduced water (AWE), compared to 1.157 mg/g for WE and 2.215 mg/g for EE. Improvement was caused by better and more elution of both hydrophobic and hydrophilic substances using alkaline reduced water and also by a synergistic effect between the antioxidant and anti-inflammatory activities of AWE extracts.
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Lee MJ, Oh JS, Park JW, Kim JK, Choi DY, Kim CH. Antioxidant activity of extract from Scutellaria baicalensis Georgi. J. Life. Sci. 10: 236–240 (2000)
Yoon HJ, Park YS. Effects of Scutellaria baicalensis water extract on lipid metabolism and antioxidant defense system in rats fed high fat diet. J. Korean Soc. Food Sci. Nutr. 39: 219–226 (2010)
Chi TS, Lim H, Park H, Kim HP. Effects of wogonin, a plant flavone from Scutellaria radix on skin inflammation: In vivo regulation of inflammation-associated gene expression. Biochem. Pharmacol. 66: 1271–1278 (2003)
Gao D, Tawa R, Masaki H, Okano Y, Sakurai H. Protective effects of baicalein against reactive oxygen species. Chem. Pharm. Bull. 46: 1383–1387 (1998)
Huang Y, Tsang SY, Tao X, Lau CW, Su YL, Chen ZY. Baicalein induced vascular response in rat mesenteric artery: Role of endothelial nitric oxide. Clin. Exp. Pharmacol. Physiol. 29: 721–724 (2002)
Kimura Y, Kubo M, Tani T, Arichi OH. Studies on Scutellariae Radixs: Effects on lipid peroxidation in rat liver. Chem. Pharm. Bull. 29: 2610–2617 (1981)
Choi WK, Pi SJ, Kim NS, Woo JK, Kim KS, Cho TH. Caffeine dissolution properties of coffee beans in electrolyzed alkaline water. Korean J. Waters 1: 25–29 (2010)
Lee KJ. Alkaline reduced water. Funct. Waters 1: 21–26 (2010)
Kang SC, Seo HJ. Storage enhancement of citrus fruits by washing treatment with electrolyzed alkaline water. Life Sci. Res. 1: 149–160 (2002)
Choi WK. Serpentine pretreatment using electrolyzed reduced water for mineral carbonation materials. Trans Korean Hydrog. New Energy Soc. 20: 447–454 (2009)
Nocco PB. Mineral water as a cure. Veroff. Schweiz. Ges. Gesch. Pharm. 29: 13–402 (2008)
Behzadi G, Fekri L. Electrical parameter and permittivity measurement of water samples using the capacitive sensor. Int. J. Water Resour. Environ. Sci. 2: 66–75 (2013)
Shirahata S, Kabayama S, Nakano M, Miura T, Kusumoto K, Gotoh M, Hayashi H, Otsubo K, Morisawa S, Katakura Y. Electrolyzedreduced water scavenges active oxygen species and protects DNA from oxidative damage. Biochem. Bioph. Res. Co. 234: 269–274 (1997)
Sim K, Shin H, Youn HJ, Lee HL. Isolation and purification of hemicelluloses in alkali pre-extractives from mixed hardwood. J. Korea Tech. Assoc. Pulp Pap. Ind. 42: 64–70 (2010)
Lee HS, Kweon MH, Lim WJ, Sung HC, Yang HC. An anticoagulant polysaccharide isolated from the alkali extracts of Coriolus versicolor. Korean J. Food Sci. Technol. 29: 369–375 (1997)
Lee AY, Choo BK, Chun JM, Kim HJ, Jang S, Lee HY, Kim HS. Quality monitoring of distributed herbal medicine, Scutellariae Radix. Korean J. Med. Crop Sci. 16: 150–154 (2008)
Kim CH, Kwon MC, Han JG, Na CS, Kwak HG, Choi GP, Park UY, Lee HY. Skin-whitening and UV-protective effects of Angelica gigas Nakai extracts on ultra high pressure extraction process. Korean J. Med. Crop Sci. 16: 255–260 (2008)
Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J. Immunol. Methods 65: 55–63 (1983)
Dietz BM, Kang YH, Liu G, Eggler AL, Yao P, Chadwick LR, Pauli GF. Xanthohumol isolated from humulus lupulus inhibits menadioneinduced DNA damage through induction of quinone reductase. Chem. Res. Toxicol. 18: 1296–1305 (2005)
Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS, Tannenbaum SR. Analysis of nitrate, nitrate, and [15N]nitrate in biological fluids. Anal. Biochem. 126: 131–138 (1982)
Shen YC, Chiou WF, Chou YC, Chen CF. Mechanisms in mediating the anti-inflammatory effects of baicalin and baicalein in human leukocytes. Eur. J. Pharmacol. 465: 171–181 (2003)
Shieh DE, Liu LT, Lin CC. Antioxidant and free radical scavenging effects of baicalein, baicalin and wogonin. Anticancer Res. 20: 2861–2865 (2000)
Nagai H, Osuga K, Koda A. Inhibition of hypersensitivity reactions by soluble derivatives of baicalein. Jpn. J. Pharmacol. 25: 763–772 (1975)
Ju MJ, Do JR, Kwon JH, Kim HK. Physiological activities of mistletoe extracts from Viscum album L. J. Korean Soc. Food Sci. Nutr. 38: 529–534 (2009)
Kim SC, Ahn KS, Park CK, Jeon BS, Lee JT, Park WJ. Isolation of antioxidative compound from Scutellaria baicalensis G. Korean J. Med. Crop Sci. 14: 212–216 (2006)
Lin YL, Lin JK. (-)-Epigallocatechin-3-gallate blocks the induction of nitric oxide synthase by down-regulating lipopolysaccharideinduced activity of transcription factor nuclear factor-kappaB. Mol. Pharmacol. 52: 465–472 (1997)
Tsai SH, Lin-Shiau SY, Lin JK. Suppression of nitric oxide synthase and the down-regulation of the activation of NF kappaB in macrophages by resveratrol. Brit. J. Pharmacol. 126: 673–680 (1999)
Liang YC, Huang YT, Tsai SH, Lin-Shiau SY, Chen CF, Lin JK. Suppression of inducible cyclooxygenase and inducible nitric oxide synthase by apigenin and related flavonoids in mouse macrophages. Carcinogenesis 20: 1945–1952 (1999)
Wright RJ. Stress and atopic disorders. J. Allergy Clin. Immunol. 116: 1301–1306 (2005)
Heo JH, Song HN, Jang SI. Effects of gagambojungikgi-tang on the immune mediators regulation and wound healing in the rsj26 or chemical antigen induced atopic dermatitis model mice. Korean J. Herbol. 23: 53–61 (2008)
Lee DJ, Jeon IH, Kim HS, Cho IY, Jang SI. Antioxidative and antiinflammatory effect of ethanol extract from duchesneachrysantha. Kor. J. Ori. Physiol. Pathol. 26: 59–66 (2012)
Lai MY, Chen CC, Hsiu SL, Chao PD. Analysis and comparison of baicalin, baicalein and wogonin contents in traditional decoctions and commercial extracts of Scutellariae Radix. J. Food Drug Anal. 9: 145–149 (2001)
Rhee JS, Woo ER, Kim NH, Lee EJ, An DK, Lee JH, Park SK, Park HK. A Study on qualitative and quantitative analysis of major ingredients in Scutellariae radix. J. Anal. Sci. Technol. 10: 91–104 (1997)
Tomimori T, Jin H, Miyaichi Y, Toyofuku S, Namba T. Quantitative analysis of flavonoids in Scutellaria roots by high-performance liquid chromatography. J. Pharm. Soc. Jpn. 105: 148–155 (1985)
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Choi, W., Kwon, HS. & Lee, H.Y. Enhancement of anti-skin inflammatory activities of Scutellaria baicalensis using an alkaline reduced water extraction process. Food Sci Biotechnol 23, 1859–1866 (2014). https://doi.org/10.1007/s10068-014-0254-6
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DOI: https://doi.org/10.1007/s10068-014-0254-6