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Effect of sodium hypochlorite (NaClO) sanitizer-induced stress on growth kinetics and morphological changes in Escherichia coli and Bacillus cereus spores

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Abstract

Sanitizer-induced stress on the growth kinetics of Escherichia coli and Bacillus cereus spores was investigated. Morphologies of B. cereus vegetative cells and spores were also compared. Nonpathogenic E. coli and pathogenic E. coli O157:H7 and B. cereus spores were treated with 100 ppm sodium hypochlorite in inoculated broth and stored at 13, 18, 24, 30, and 36°C. The lag time (LT) of stressed E. coli was extended more at low temperatures, regardless of pathogenicity. E. coli and B. cereus vegetative cells were sensitive to a sanitizer treatment with NaClO. Stressed strains grew more rapidly than unstressed strains. However, sanitizer stress did not significantly (p>0.05) affect the LT and specific growth rate (SGR) of B. cereus spores, regardless of storage temperature. Transmission electron microscopy analysis also revealed that stress induced using the NaClO sanitizer caused morphological damage to B. cereus vegetative cells, but not to B. cereus spores.

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Authors and Affiliations

  1. Department of Food and Nutrition, Kyung Hee University, Seoul, 130-701, Korea

    Soo Jin Kwak, Hye Jin Jo & Ki Sun Yoon

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  1. Soo Jin Kwak
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  2. Hye Jin Jo
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  3. Ki Sun Yoon
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Correspondence to Ki Sun Yoon.

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Kwak, S.J., Jo, H.J. & Yoon, K.S. Effect of sodium hypochlorite (NaClO) sanitizer-induced stress on growth kinetics and morphological changes in Escherichia coli and Bacillus cereus spores. Food Sci Biotechnol 23, 815–821 (2014). https://doi.org/10.1007/s10068-014-0110-8

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  • DOI: https://doi.org/10.1007/s10068-014-0110-8

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