Abstract
Denitrification is a key process buffering the environmental impacts of agricultural nitrate loads but, at present, remains the least understood and poorly quantified sink in nitrogen budgets at the watershed scale. The present work deals with a comprehensive and detailed analysis of nitrogen sources and sinks in the Burana–Volano–Navigabile basin, the southernmost portion of the Po River valley (Northern Italy), an intensively cultivated (> 85% of basin surface) low-lying landscape. Agricultural census data, extensive monitoring of surface–groundwater interactions, and laboratory experiments targeting N fluxes and pools were combined to provide reliable estimates of soil denitrification at the basin scale. In the agricultural soils of the basin, nitrogen inputs exceeded outputs by nearly 40% (~ 80 kg N ha−1 year−1), but this condition of potential N excess did not translate into widespread nitrate pollution. The general scarcity of inorganic nitrogen species in groundwater and soils indicated limited leakage and storage. Multiple pieces of evidence supported that soil denitrification was the process that needed to be introduced in the budget to explain the fate of the missing nitrogen. Denitrification was likely boosted in the soils of the studied basin, prone to waterlogged conditions and consequently oxygen-limited, owing to peculiar features such as fine texture, low hydraulic conductivity, and shallow water table. The present study highlighted the substantial contribution of soil denitrification to balancing nitrogen inputs and outputs in agricultural lowland basins, a paramount ecosystem function preventing eutrophication phenomena.
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Highlights
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A detailed N budget in soils and waters was calculated for a lowland coastal basin.
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N inputs in agricultural soils exceeded outputs by nearly 40%.
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Waterlogged condition in fine-texture soils promoted NO3− removal via denitrification.
Introduction
In the last century, the intensification of agricultural activities has deeply altered the nitrogen (N) budgets in the cropping systems of agriculturally dominated watersheds around the world. The N amount added to croplands through fertilization in excess to crop demand is lost to the environment, in particular, surface and groundwater, with multiple detrimental implications in terms of, for example, water quality deterioration, human health, and biodiversity loss (Erisman and others 2011; Houlton and others 2019). Nitrate (NO3−) release from diffuse agricultural sources is still an unsolved environmental threat, a cause of eutrophication and groundwater pollution (Glibert and others 2006; Bodirsky and others 2014; Leip and others 2015).
Many questions on the fate of the missing N in agriculturally dominated basins remain open. For instance, about only 25%, on average, of the N load generated within worldwide watersheds is delivered to terminal waterbodies and coastal zones via river export, but still little is known about the relative importance of different N retention mechanisms (Howarth and others 2012; Goyette and others 2016; Romero and others 2016). On this basis, questions on where and for how long N may accumulate in soils and aquifers and what transformations and removal processes it may undergo, preventing its export, are not yet properly addressed.
Heterotrophic denitrification, the microbial anaerobic respiration that reduces NO3− to dinitrogen gas (N2), is globally considered the main biogeochemical reaction by which bioreactive N is lost to the atmosphere (Kulkarni and others 2008; Bouwman and others 2013). In aquifer systems, denitrification can occur also by oxidation of iron–sulfur minerals such as pyrite (Bosch and others 2012; Jessen and others 2017). In fertilized fine-textured soils, rainfall and poor drainage are expected to increase denitrification by decreasing oxygen availability and making the soil matrix more and more hypoxic and, thus, more prone to anaerobic respiration pathways (Hofstra and Bouwman 2005; Kuzyakov and Blagodatskaya 2015). Understanding if NO3− accumulates in the vadose zone or is permanently removed by denitrification (and where) is a key element for designing and implementing effective land use management strategies that protect water quality in agricultural basins (Wang and others 2017; Xin and others 2019; Kolbe and others 2019). In budgeting N in large agricultural areas, the main difficulty is to consider a multiplicity of N storages and sinks. Some terms, such as NO3− storage in the vadose zone (Ascott and others 2017; Van Meter and others 2016; Xin and others 2019) and artificial canal networks acting as N metabolic regulator in irrigated landscapes (Dollinger and others 2015; Soana and others 2019; Goeller and others 2020), have been assessed by an increasing number of studies in the last years, but their contribution to N removal at the watershed scale is case-specific. Moreover, heterogeneous conditions in terms of N fertilization practices (that is, type and dosage of fertilizers, application methods, and timing), hydrological processes, and soil types may result in an extreme increase of site-specificity, both in the generation and transformation of N loads. Denitrification in agricultural soils remains the least understood and poorly quantified sink in N budgets at the basin scale (Davidson and Seitzinger 2006; Almaraz and others 2019; De Girolamo and others 2019).
Conventional biogeochemical techniques (for example, acetylene inhibition method, 15N tracer methods) based on the incubation of intact soil cores, mesocosms, or slurries have long been adopted to measure soil denitrification and have contributed to the understanding of proximal drivers of the process, such as temperature, NO3−, and organic carbon availability (Barton and others 1999; Hofstra and Bouwman 2005; Chalk and others 2019). However, high uncertainty arises in extrapolating instantaneous point measurements to broader scales, as denitrification rates vary greatly in space and time, not only among different agroecosystems but also within the same parcel. Sampling and modeling approaches generally do not properly account for those small areas (hotspots) and brief periods (hot moments) that express the highest denitrification rates and remove a disproportionately high NO3− amount, causing a large underestimation of the overall denitrification relevance (Groffman and others 2009; Anderson and others 2014; Kuzyakov and Blagodatskaya 2015). On the other hand, modeling tools and geospatial approaches may also generate large overestimates when based on the upscaling of potential rates measured under optimal conditions of NO3− and organic carbon availability for denitrifying bacteria and not at all representative of in situ activity (Oehler and others 2009). Due to the complexity of field measurements, soil denitrification is rarely addressed as the main focus of research dealing with watershed N budgets. Nevertheless, where a huge amount of data (for example, high-resolution agricultural statistics, extensive monitoring of surface waters and aquifers, laboratory experiments targeting N fluxes and pools in soils, and so on) is available and the N inputs, outputs, and storages in croplands are accurately quantified, the mass balance approach may provide a reliable basin-level estimate of soil denitrification and a benchmark value to validate field measurements.
The present study aims at a better understanding of the watershed-scale importance of soil denitrification as a NO3− buffering mechanism in agricultural settings. A comprehensive and detailed analysis coupling N budget in the soil-shallow aquifer system and in surface waters allowed us to explain the fate of N excess by disentangling denitrification from groundwater storage and by also partitioning the contribution of soils and canal sediments in removing NO3− via denitrification.
The Burana–Volano–Navigabile basin, the southernmost portion of the Po River valley (Northern Italy), was selected as a case study for three main reasons: (1) the territory is intensively cropped and characterized by fine-textured soils; (2) the basin is completely flat and lies for the most part below the sea level and, thus, all water inputs and outputs are completely artificially managed and regularly monitored, allowing accurate water and N budgets in the canal network; (3) extensive information is available on hydrogeological drivers and bacterial processes controlling N transformations and horizontal and vertical migration paths. Soil features regulating NO3− removal have been deeply investigated in laboratory-controlled experiments (Castaldelli and others 2019; Mastrocicco and others 2019a), and preliminary estimates of basin-scale soil denitrification rates were obtained in the deltaic portion of the Po River Basin through a detailed investigation about N sources and sinks in the croplands (Castaldelli and others 2020). A comprehensive and quantitative understanding of denitrification in the context of N fluxes at the basin scale is still lacking, and there is an urgent need to integrate all the acquired findings to shed light on this still missing pathway of the N cycle in agriculturally dominated landscapes. The main novelty of this work is to present a basin-level estimate of soil denitrification to gain insight into its relevance as a nitrate buffering mechanism where agriculture deeply affects watershed N budgets.
Materials and Methods
Study Area
The Burana–Volano–Navigabile watershed (~ 2600 km2, Figure 1) is a deltaic territory, recently reclaimed and intensively cropped, approximately overlapping the administrative borders of the Ferrara Province (Emilia-Romagna Region, Northeastern Italy). This basin is the terminal portion of the Po River Basin (Figure 1a, b), the largest hydrographic system in Italy and one of the most agriculturally exploited and densely populated areas in Europe. Here, eutrophication and NO3− pollution of surface and groundwater are serious environmental issues (Viaroli and others 2018; Martinelli and others 2018; Malagó and others 2019; Musacchio and others 2020). The Burana–Volano–Navigabile watershed covers an area extending from the city of Ferrara to the Adriatic Sea and is limited to the north by the Po River main course and to the south by the Reno River. The climate is warm temperate (Type Cfa, according to Köppen–Geiger classification, Beck and others 2018) with a mean wet deposition of about 600 mm year−1 over the last 20 years (data from seven weather stations belonging to the official monitoring network of the Hydro-Meteorological Service of the Environmental Protection Agency, Emilia-Romagna Region; https://simc.arpae.it/dext3r/). According to the World Reference Base for Soil Resources classification (IUSS Working Group WRB 2006), the soil textures are: silty-loam (42% of the basin area), silty-clay (26%), peat (23%), and sand (9%).
More than 85% of the basin surface is devoted to agriculture (that is, utilized agricultural area—UAA), fertilized almost exclusively with synthetic urea and ammonium nitrate, and about 10% is classified as artificial area with a spread urban planning scheme characterized by low population density (Figure 1d). Cereals are the main crops, covering more than half of the agricultural land (wheat, maize, and rice, 30%, 21%, and 4% of the UAA, respectively), followed by industrial and horticultural crops (22%) and feed crops, mostly alfalfa (10%), and fruit trees (9%). Animal husbandry consists of poultry (1.4 × 106), pigs (~ 48,000), and cattle (~ 22,000), resulting in < 0.5 livestock unit—equivalent to an adult dairy cow—per ha of UAA (National Statistics Institution, 6th Agricultural Census 2010; http://dati-censimentoagricoltura.istat.it). The whole Burana–Volano–Navigabile watershed was declared a Nitrate Vulnerable Zone (NVZ) from agricultural sources, in accordance with the European Nitrates Directive (91/676/EEC) and the NVZ Action Plan of the Emilia-Romagna Region.
This basin lies in a depressed area for the most part below the sea level (on average, 2 m a.s.l.); thus, the hydrological regime is artificially regulated by a capillary network of canals, pumping stations, and gates. Irrigation water (500–2500 × 103 m3 day−1 from April to September) is derived from the Po River and feeds several open-earth canals surrounding fields (linear extension of 3965 km, Figure 1c). The main irrigation systems through which the Po River water is supplied to the agricultural activities are aspersion and microirrigation, used in 51% and 21% of the irrigated surface, respectively (National Statistics Institution, 6th Agricultural Census 2010; http://dati-censimentoagricoltura.istat.it). Excess water is drained from the fields and discharged through some major collectors into the Po di Volano, an ancient branch of the Po River Delta at present canalized (Cencini 1999). The shallow unconfined aquifer, whose thickness ranges between 2 and 7 m, is fed by both rain infiltration and surface water system; thus, the water table usually fluctuates between 0.5 and 2.0 m from the ground surface (Mastrocicco and others 2017).
Calculation of N Budget in Soils
The N budget in soils was compiled for the 5-year period 2006–2010 by employing statistical data on agricultural activities and experimental measurements in soils and waters (that is, field monitoring campaigns and laboratory experiments) collected thanks to several research projects performed in the Burana–Volano–Navigabile watershed. N inputs and outputs across the UAA were computed according to the methodology developed by Castaldelli and others (2020) and previously tested and applied in a portion of the Po River Delta. The N budget approach adopted here got its robustness from the use of high-spatial-resolution datasets (for example, crop areas, livestock densities), site-specific agronomic coefficients (for example, crop-specific fertilizer recommendations, crop yields), and N cycling measurements gathered from the study area (for example, ammonia volatilization rates, rainfall N content). Official agricultural census data, the basis for all agriculture regulations in Italy, were provided by local (Ferrara Province, Agriculture Department) and national (National Statistics Institution) statistical authorities. Moreover, for the study area, statistical data and agronomic coefficients used to compile the budget were checked and validated by a panel of 30 agronomists who worked as Ferrara Province consultants in the framework of the EC project EU-WATER (“Transnational integrated management of water resources in agriculture for the European WATER emergency control,” contract n. SEE/A/165/2.1/X; http://www.eu-water.eu/).
N inputs and outputs were first calculated for each of the 26 municipalities (surfaces from 16 to 440 km2) including the Burana–Volano–Navigabile watershed and then aggregated at the basin scale. According to the official EU division for regional statistics elaborated by EUROSTAT (2015), Italian municipalities correspond to the LAU-2 territorial level, Italian provinces to the NUTS-3 territorial level, and Italian regions to the NUTS-2 territorial level. Municipality is the smallest administrative unit at which official agricultural and demographic statistics are usually available for the whole national territory and also the scale at which the Nitrates Directive restrictions are applied.
The N budget in soils was calculated as follows:
where Man = N in livestock manure applied to UAA, Synth = N in synthetic N fertilizers applied to UAA, Fix = N from biological fixation, Dep = N deposition on UAA, Harv = N exported from UAA by crop harvest, Vol = N lost to the atmosphere by ammonia (NH3) volatilization, Runoff = N lost from agricultural soils to surface water via runoff, and Den(s) = denitrification in soils.
A detailed description of data sources, budget equations, and uncertainty assessment is reported in Supplementary Materials 1. A Microsoft Excel spreadsheet containing the formulae for the computation of each budget term of Eq. 1 was employed. N inputs and outputs were spatialized at the municipality level by GIS analysis (QGIS software 2.18, https://www.qgis.org/it/site/).
N lost from agricultural soils to surface water via runoff (Runoff) was estimated by difference after all the other terms of the N budget in the canal network were quantified according to Eq. (2), as detailed in the following paragraph. The unaccounted complement of the balance in soils, that is, the difference between input and other loss terms was assumed to be ascribed to denitrification (Den(s)). This assumption was supported by the provision of data proving a general scarcity of mineral N forms in both shallow aquifers and soils. The outcomes from the extensive monitoring of the shallow aquifer were integrated with those obtained by previous repeated analytical campaigns in surficial soils of the investigated basin (Castaldelli and others 2013a; Mastrocicco and others 2019b) to assess the magnitude of N-reactive forms’ availability in the vadose zone.
Spatiotemporal nitrate contamination in the shallow aquifer was assessed by analyzing the outcomes from a 2-year sampling campaign. A network of 56 monitoring wells was installed throughout the agricultural land of the Burana–Volano–Navigabile watershed covering the predominant soil textures and main crop (Figure 1e). The monitoring wells screens (20 cm long) were located just below the shallow water table (on average, at 2 m below ground level) to capture the recharge water pulses that reached the aquifer. Sampling campaigns were performed almost monthly in 2010 and 2011 to evaluate N species distributions in the shallow unconfined aquifer. After purging at least two times the well volume, water samples were collected using an inertial pump, filtered in situ through 0.22 µm polypropylene filters and stored at 4 °C during the transport to the laboratory. Here, samples were analyzed for NO3− using Technicon AutoAnalyser II (Armstrong and others 1967).
Calculation of N Budget in the Canal Network
The N budget in the canal network was compiled by employing statistical data on population and industrial activities and field observations collected in the 5-year period 2006–2010. According to the methodology developed by Castaldelli and others (2020), the following equation was used:
where Inflow = N load imported in the watershed by the canal network, Urb = N load generated in urban areas, Dep_Urb = N deposition on surfaces other than agricultural land (that is urban and industrial areas), Ind = N load generated in industrial areas, Runoff = N lost from agricultural soils to surface water via runoff, Outflow = N load exported from the watershed by the canal network, and Den(c) = N removed in the canal sediments by denitrification.
A description of the calculation of N budget in the canal network is detailed in Supplementary Materials 1.
Results
N Budget in Soils
Within the 5-year period 2006–2010, the N budget in the agroecosystems of the Burana-Volano-Navigabile watershed evidenced that synthetic fertilizers were the main contribution to all the N imported to agricultural soils in the basin, averaging at 68% of total N inputs, followed by biological fixation (23%), livestock manure (6%), and atmospheric deposition (3%) (Table 1). The proportion among the N input terms at the watershed scale reflected what was evidenced at the municipal level, with synthetic fertilizers being always the most relevant N source in all the municipalities (range 103–180 kg N ha−1 year−1), while biological fixation and livestock manure reached maximum values of 130 and 61 kg N ha−1 year−1, respectively. N from livestock manure was produced mainly by poultry breeding (43%), followed by cattle breeding (37%). Alfalfa contributed almost two-thirds of the total N input from biological fixation (Table 1). Total N input in the municipalities of the studied area varied from 163 to 274 kg N ha−1 year−1 (Figure S4, Supplementary Materials 2), with an average value of 205 kg N ha−1 year−1 at the basin scale (Table 1). N export via crop harvest was the main output from soils among the three terms included in the budget, accounting for about 97% of the sum of all items (Table 1). Amount of N removed from soils with crop harvest (municipal values ranging from 103 to 160 kg N ha−1 year−1) was two orders of magnitude higher than that removed through other loss pathways, that is, runoff and NH3 volatilization. Two cereals (maize and wheat) accounted for more than 50% of the total N removed in crop biomass from cultivated land. Total N output in the municipalities of the studied area varied from 104 to 163 kg N ha−1 year−1 (Figure S4, Supplementary Materials 2), with an average value of 123 kg N ha−1 year−1 at the basin scale (Table 1).
After accurately accounting for all the budget terms, however, inputs (~ 37,500 t N year−1) exceeded outputs (~ 22,600 t N year−1) by about 40% (Table 1). At the basin scale, the average unaccounted term was 81 kg N ha−1 year−1, with municipal values ranging from 47 to 128 kg N ha−1 year−1 (Figure S4, Supplementary Materials 2).
Nitrate Concentrations in Surface Waters and Shallow Aquifer
During the investigated 5-year period, NO3− concentrations of the water volumes entering the canal network displayed a marked seasonal variation (Figure 2a), with the highest values recorded in the middle of the winter (January, median value 3.7 mg N L−1) and minimum values in the middle of the summer (July, median value 1.3 mg N L−1). Nitrate decreased constantly from January to July, with values that more than halved along the first 7 months of the year and then increased again in the second part of the year. Median NO3− concentrations of the water volumes exiting the canal network remained in the range 2.0–3.0 mg N L−1 from January to April and showed values constantly lower than 1 mg N L−1 from June to October, before increased again starting from the middle of the autumn (Figure 2b). For each month, NO3− concentrations of the water exiting the basin were systematically lower, despite the higher variability, than those conveyed by the Po River water imported into the canal network (Figure 2a, b), up to over 75% in the late summer.
During the whole monitoring period, NO3− concentrations in the shallow aquifer were generally much lower than those measured in surface waters and without any clear seasonal trend: median monthly values never exceeded 0.2 mg N L−1, and only in some transient occasions did NO3− concentrations peak at 4–7 mg N L−1 (Figure 2c). In all sites and months, NO3− was constantly below the limit of 11.3 mg N L−1, the maximum permissible concentration in drinking water according to the World Health Organization (WHO 2004). The concentrations were quite homogeneous at the spatial scale, with only a few hotspots in correspondence to groundwater samples belonging to sandy soils (that is, Po River paleo-channels and coastal paleo-dunes) (Figure 3).
N Budget in the Canal Network
The canal network imported annually about 550 t N via irrigation waters, with monthly N loads ranging from minimum values in late autumn–winter period (< 10 t N month−1) to maximum values in late spring–summer period (up to 117 t N month−1 in July) (Figure 4). Monthly N loads exported from the basin followed an inverse seasonal pattern with summer minima (18–35 t N month−1) and winter peaks (up to 186 t N month−1 in December) for a total of about 1000 t N reaching annually the coastal area via drainage waters. The canal network acted as an N sink throughout the summer months (from June to September), with a net decrease between imported and exported N loads ranging from 15 to 95 t N month−1 (Figure 4). During the whole summer, N loads reaching the coastal area were lower by 38–85% than those imported into the canal network and from upstream ecosystems. In contrast, during the rest of the year, monthly N loads exported by the canal network were 4 to almost 30 times greater than the corresponding imported N loads, with the maximum increase detected in winter.
N removal via denitrification in the canal network of the basin was estimated as 740 t N year−1 (Figure 5) of which about 73% occurred from April to September when the canals were active for irrigation. Vegetated canals accounted for only a minor fraction (7–11%) of the total removal for the months from April to June when the emergent vegetation can develop prior to mowing operations carried out by the local water management authority. The highest monthly N removal rates via denitrification in the canal network were estimated in the spring–summer period (68–125 t N month−1) when also the maximum monthly differences between imported and exported N loads were recorded (Figures 4, 5).
N loads generated in urban and industrial areas and discharged into the canal network were estimated as about 570 t N year−1 (~ 334,000 inhabitants) and about 200 t N year−1, respectively (Figure 6). N load imported from upstream ecosystems and N loads generated in urban areas were the two main inputs in the budget in the canal network and contributed almost equally (overall 65%), followed by N deposition on surfaces other than agricultural soils (24%), N load from industrial areas (8%), and runoff (3%). Point N loads generated in urban and industrial areas (~ 1100 t N year−1) corresponded as a whole to less than 3% of the diffuse N loads from agricultural activities (that is, total N input to agricultural soils, ~ 37,500 t N year−1) (Figure 6).
Discussion
The detailed N budget in the agroecosystems and canal network of the Burana–Volano–Navigabile watershed showed that: (1) N inputs in agricultural soils exceeded outputs by nearly 40% (that is, on average ~ 80 kg N ha−1 year−1); (2) N runoff to surface waters accounted for less than 1% of the total N input to soils; (3) even though the magnitude of N input to soils was very large, NO3− concentrations recorded in the shallow aquifer of the whole watershed were steadily very low; (4) canal network acted as a net NO3− sink during the spring–summer months by attenuating the N load imported with irrigation water; and (5) denitrification in soils was likely the process responsible for balancing N inputs and outputs.
N Budget in the Agroecosystems of the Burana–Volano–Navigabile Watershed
The areal N input through fertilization in the Burana–Volano–Navigabile watershed (range 163–274 kg N ha−1 year−1) was among the highest reported for European watersheds (Vagstad and others 2004; Hou and others 2015; Lassaletta and others 2016; Poisvert and others 2017) and suggested a condition of potential pollution for aquatic ecosystems. Nitrogen use efficiency, that is, the proportion of all N inputs removed from arable lands via crop harvest, reached about 60%, which is higher than the values obtained for other agricultural sub-basins of the Po River system, such as Oglio and Mincio rivers (range 35–50%) (Viaroli and others 2018; Racchetti and others 2019; Pinardi and others 2020). The difference lies in the prevalent use, in the basin studied here, of synthetic fertilizers, more accurately dosable than manure, prevalently used in the Oglio and Mincio basins, whose excess may generate greater losses to surface and groundwater (Severini and others 2020). However, although the distribution of synthetic fertilizers allows crop demand to be met more accurately, owing to their rapid dissolution and transformations in soils (Castaldelli and others 2018), it may increase the risk of lateral or vertical NO3− transport. As the study basin is a completely flat territory with a surface slope mostly less than 0.05%, the fraction of N excess reaching the canal network via surface runoff resulted in a negligible term of the N budget along the terrestrial–freshwater continuum. This is coherent to the outcomes of runoff modeling that have previously estimated a N loss of on average about 3 kg N ha−1 year−1 for the investigated basin (Aschonitis and others 2013). On the other hand, in these conditions, vertical transport through the vadose zone may be predominant, and the aquifer is exposed to a high risk of NO3− pollution.
Relationships between N Inputs in Soils and N Export to the Coastal Zone
On an annual basis, the Burana–Volano–Navigabile watershed exports to the coastal zone an N load that almost doubles the load entering in the canal network from the Po River and the basins located upstream. This increase (~ 440 t N year−1) represents less than 3% of the missing N in soils (~ 14,800 t N year−1) and could be explained by urban point sources. Moreover, the canal network acted as an effective N sink in the spring–summer months when a relevant portion (~ 32% along the whole irrigation period, from April to September) of the N load, generated by upstream agroecosystems and imported with irrigation water, was removed by denitrification at the sediment level, protecting the coastal area, when the risk of eutrophication is higher. Drainage canals, at present, are almost completely unvegetated, and the adoption of more conservative management practices to restore emergent macrophytes would contribute to a further increase in the denitrification potential. Studies done in the same hydrological network have evidenced a tenfold increase of the N removal via denitrification in the presence of emergent vegetation, such as Phragmites australis and Typha latifolia, compared with unvegetated canals (Pierobon and others 2013; Soana and others 2019).
The annual N export to the coastal zone corresponds to nearly 3% of the total N input generated in the agroecosystems, a value that is much lower than the range (7–20%) reported for other agricultural sub-basins of the Po Plain (Bartoli and others 2012; Pinardi and others 2020) and for the same Po River Basin as a whole (Viaroli and others 2018). Nevertheless, a high retention capacity may be due to both permanent and temporary removal processes, such as denitrification in soils and storage in the vadose zone–groundwater system. This is what has been evidenced in other Po River sub-basins, where a combination of N excess, mainly due to manure spreading, and flood-based irrigation practices over permeable coarse soils, promoted NO3− leaching and accumulation into groundwater (Musacchio and others 2020; Racchetti and others 2019). Differently, for the Burana–Volano–Navigabile, a ubiquitous and constant scarcity of inorganic N forms has been evidenced not only in the shallow aquifer, as reported here, but also in surficial soils where only NO3− was found in the range of 10–35 kg N ha−1 year−1 as demonstrated by extensive monitoring campaigns (Castaldelli and others 2013a; Mastrocicco and others 2019b).
For the dominant soil textures, silty-loam and silty-clay, covering more than two-thirds of the arable land surface and intensively fertilized mainly with synthetic urea, organic matter content is on average less than 3% (Castaldelli and others 2013a). Thus, the contribution of N storage in soil organic matter is likely irrelevant in the investigated basin. Here, the supply of organic matter in the form of manure is low because of low livestock density, and only sandy soils, covering less than 10% of the territory, are amended with chicken manure whose mineralization is very fast. In the deltaic portion of the Po River lowlands, livestock density underwent a constant decline in the last four decades (Viaroli and others 2018); thus, in the long term, negligible organic fertilization promoted the depletion of soil organic pools (Natali and others 2018). These outcomes indicate that soil and groundwater storage is unlikely to explain the discrepancy between N input and output in agricultural land. On the contrary, this evidence, together with the multiple terms accounted in the N budget, strongly supports the hypothesis that the large missing N has been permanently removed from the system via soil denitrification.
The Role of Soil Denitrification as N Sink in Agricultural Watersheds
In soils, the simultaneous presence of hypoxic/anoxic conditions, NO3−, and labile organic carbon availability are triggering conditions for the anaerobic respiration of NO3− by heterotrophic denitrifying bacteria (Tiedje 1988; Wallenstein and others 2006). By affecting redox status, soil water content is considered a key driver for N removal via denitrification, and the chance for creating anoxic microsites is higher in soils with high porosity, dead-end pores, and greater water retention (clayey and loamy soils) than in more permeable coarsely textured soils (Barton and others 1999; Barakat and others 2016). Thus, in the Burana–Volano–Navigabile watershed, characterized by soils with fine textures, denitrification may find suitable conditions due to very low hydraulic conductivity, flat topography, and shallow water tables, all features that, in the occasion of intense rainfall, promote the establishment of waterlogged conditions and, consequently, oxygen shortage (Mastrocicco and others 2013, 2017).
In temperate regions, agricultural soils are commonly characterized by hypoxic conditions since they remain generally wet along the winter–spring months and, in the case of water table proximal to the ground surface, as in the studied basin, occasionally completely waterlogged after heavy rainfalls. These represent the moments of potentially higher N loss via denitrification due to NO3− accumulation in soil solution when crop residues are degraded by soil microorganisms or after fertilizer applications that are usually performed in coverage for the most N-demanding crops (for example, wheat and maize). The present findings are in full agreement with multiple pieces of evidence from laboratory studies, performed in the same soils, which indicated high NO3− consumption and correspondent N2 production in mesocosms under water-saturated conditions (Mastrocicco and others 2011, 2019a; Castaldelli and others 2019). Nitrate reduction could be also due to pyrite oxidation that could concur to remove excess NO3− in reducing environments low in organic carbon substrates (Jessen and others 2017). Moreover, dissimilative NO3− reduction to ammonium cannot be excluded as a relevant process contributing to NO3− removal in organic-rich sediments such as rice fields (Putz and others 2018; Pandey and others 2019), though covering less than 10% of total agricultural land in the basin. The present outcomes are consistent with preliminary estimates of basin-scale N budget in the deltaic portion of the Po River highlighting an average unaccounted term of about 70 kg N ha−1 year−1 likely attributable to soil denitrification (Castaldelli and others 2020). The investigated basin encompasses all the representative soil types and crop types of the Po River lowlands; thus, the findings and the developed approach can be easily exported to other watersheds with similar features and climates in the Mediterranean area, Europe, or worldwide.
Labile organic carbon availability is a key factor supporting the denitrifying capacity and its role in protecting groundwater from diffuse NO3− pollution. The presence of low-molecular-weight organic acids such as acetate was demonstrated to deeply affect NO3− persistence in fertilized soils with high acetate concentrations corresponding to very low NO3− content (Castaldelli and others 2013b). In the investigated soils, organic acids originating from the decomposition of crop residues and from root exudates represent often the only organic inputs, since amendment with manure is scarce. This stresses the need to adopt agronomic practices that maintain sufficient organic matter in soils to sustain their intrinsic buffer capacity against NO3− pollution.
Areal denitrification rate, calculated here by mass balance, at the basin scale on average about 80 kg N ha−1 year−1, was higher than the literature values measured in fine-textured fertilized soils during the growth period of annual crops, generally never exceeding 50 kg N ha−1 year−1 and in most cases within the range of 20–30 kg N ha−1 year−1 (Nieder and others 1989; Barton and others 1999; Hofstra and Bouwman 2005). Measurements performed with sufficient intensity in time and space to obtain robust estimates at the annual scale, that is, the temporal scale at which N budgets are generally calculated in watersheds, are still lacking. Denitrification remains one of the least well-quantified processes of N cycling in soils owing to the difficulty of quantifying it accurately by capturing its high spatial and temporal variability (Groffman and others 2009; Anderson and others 2014).
In the present study, N budget relays on robust datasets acquired in the study area, such as high-resolution crop maps, agricultural statistics, and site-specific agronomic coefficients validated by expert judgment. In such a system where all N flows and pools have been identified and accounted for, the mass balance can be successful in providing quantitative estimates of soil denitrification at the basin level, further improved since multiple years of data were used and averaged. Being calculated as a residual term, that is, the unaccounted complement of the N balance, denitrification estimate suffers from an uncertainty resulting from the uncertainty in the input data and parameters employed to compute each component of the balance. Previous studies demonstrated that atmospheric deposition and ammonia volatilization are generally among the most uncertain terms, and estimates of N losses are less accurate than inputs via fertilizers and manure (Oenema and others 2003; Zhang and others 2020). Similar outcomes were obtained here, where the coefficients of variation of these terms were among the highest (> 70%). However, atmospheric deposition and ammonia volatilization were minor terms, accounting for only 3% of the total inputs and output, respectively.
Locally derived measurements of N fluxes were also sources of uncertainty for the N budget along the terrestrial–freshwater continuum. Denitrification rates in canal sediments were measured in several canals representative of the dominant waterways. Reach-scale biogeochemical methods were applied integrating N processes occurring in multiple compartments (that is, water, sediment, vegetation, and biofilms) under natural conditions and overcoming the limits inherent in the extension of laboratory experiment outcomes to the broader scale (Soana and others 2019). Uncertainty exists in extending denitrification measures from the reach scale to landscape scale, as a consequence of the spatial and temporal heterogeneity of hydrological, geomorphological, and biological drivers that affect biogeochemical reactions. Nevertheless, despite the coefficient of variation being more than 50%, this N flux accounted overall for less than 5% of the missing N in the basin. The present analysis certainly has weaknesses that might, of course, be improved in the future, but it is a step rarely done at this scale with a combination of datasets and measurements allowing us to account for all N flows in the basin.
In conclusion, the present study strongly indicated that, under temperate subhumid climates, soil denitrification may be a relevant N sink in agricultural basins heavily fertilized and with fine-texture soils, prone to waterlogged conditions. Holistic approaches such as detailed watershed N budget, although data demanding, allow us to infer a crucial process such as soil denitrification that is, at present, still difficult to measure owing to inadequate techniques and high intrinsic variability in space and time. Future works will require accurate characterization of the seasonal trends of denitrification activity to fully prove its role in explaining the fate of missing N in lowland agricultural basins.
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Acknowledgements
This research was funded by the Emilia-Romagna Region within the Rural Development Programme (PSR) 2014–2020 (Measure 16.1.01—Operational Groups of the European Partnership for Agricultural Productivity and Sustainability), Project Ferrara Nitrates—Agricultural techniques to prevent nitrates pollution and the organic matter conservation (https://ec.europa.eu/eip/agriculture/en/find-connect/projects/nitrati-ferrara-tecniche-agronomiche-la). The authors thank the Presidents, Dr. Stefano Calderoni and Dr. Franco Dalle Vacche, and the Director, Dr. Eng. Mauro Monti, of the Ferrara Land Reclamation Consortium, and the Emilia-Romagna Agency for Environmental Protection for the kind and profitable collaboration and for having provided water flow data and water quality data, respectively.
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Soana, E., Vincenzi, F., Colombani, N. et al. Soil Denitrification, the Missing Piece in the Puzzle of Nitrogen Budget in Lowland Agricultural Basins. Ecosystems 25, 633–647 (2022). https://doi.org/10.1007/s10021-021-00676-y
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DOI: https://doi.org/10.1007/s10021-021-00676-y