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Screening of hepatitis B virus DNA in the serum sample by a new sensitive electrochemical genosensor-based Pd-Al LDH substrate

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Abstract

Screening for hepatitis B is one of the most severe liver virus infections in the world, and due to the effect of early detection and treatment of hepatitis B disease, fast and sensitive analytical devices are highly needed. A highly sensitive voltammetric genosensor is described for hepatitis B virus (HBV) detection by depositing electrochemically Pd(II)-Al(III)-layered double hydroxide (LDH) in a short time by chronoamperometry (CA) technique on the surface of the fluorinated tin oxide electrode (FTO) that is reported for the first time. The complementary DNA sequence of the amino-labeled DNA was fixed as a probe on the carboxylic group-directed LDH film. The electrochemical behavior of the designed genosensor by the attached DNA virus was investigated using the differential pulse voltammetry (DPV), cyclic voltammetric (CV), and electrochemical impedance spectroscopy (EIS) techniques in the presence of the Fe(CN)63–/4– and at the optimum conditions has a wide linear range (1 fM to 1 μM) with the experimental detection limit of 1 fM. This genosensor was successfully implemented to measure the DNA of HBV in human serum. This modified electrode has high sensitivity and short production time, which can be a candidate for the production of a portable hepatitis B diagnostic kit.

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Acknowledgements

We gratefully appreciate the excellent support of this project by the Institute Pasteur of Iran and the excellent cooperation of the Research Councils of Iran University of Science and Technology (IUST).

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Authors and Affiliations

  1. Research Laboratory of Real Samples Analysis, Faculty of Chemistry, Iran University of Science and Technology, Tehran, 1684613114, Iran

    Mozhgan Heidari & Ali Ghaffarinejad

  2. Electroanalytical Chemistry Research Center, Iran University of Science and Technology, Tehran, 1684613114, Iran

    Mozhgan Heidari & Ali Ghaffarinejad

  3. Department of Biochemistry, Genetics and Metabolism Research Group, Pasteur Institute of Iran, Tehran, 13164, Iran

    Eskandar Omidinia

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Heidari, M., Ghaffarinejad, A. & Omidinia, E. Screening of hepatitis B virus DNA in the serum sample by a new sensitive electrochemical genosensor-based Pd-Al LDH substrate. J Solid State Electrochem 26, 1445–1454 (2022). https://doi.org/10.1007/s10008-022-05176-0

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