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Effects of boric acid on alveolar sockets filling after dental extraction in rats

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Abstract

Purpose

After extraction, dental alveolus filling aims to reduce bone loss and maintain the alveolus volume during patient rehabilitation. Boric acid (BA) is a boron-derived compound with osteogenic properties and an interesting candidate for alveoli filling. This study aims to investigate the osteogenic capacity of the local application of BA in dental socket preservation.

Methods

Thirty-two male Wistar rats were submitted to upper right incisor extraction and randomly divided into four groups (n = 8): control group (no intervention), BA (8 mg/kg) socket filling, bone graft (Cerabone®, Botiss, Germany), and BA + bone graft socket filling. Animals were euthanized 28 days after dental extraction. MicroCT and histological analysis were performed to evaluate the newly formed bone on the dental alveolus.

Results

MicroCT analysis demonstrated that bone volume fraction (BV/TV), bone surface (BS), bone surface/bone volume ratio (BS/BV), bone surface density (BS/TV), trabecular thickness (Tb.Th), total bone porosity (Po-tot), and total volume of pore space (Po.V(tot)) from BA and BA + bone graft rats were significantly different from the control group. Histological evaluation displayed a delayed bone repair in BA rats, with the presence of connective tissue and inflammatory infiltrate. However, the BA + bone graft group demonstrated histological aspects like the bone graft animals, with less organized osteoblasts, suggesting inferior bone repair.

Conclusion

Osteogenic capacity did not depend on the BA local application after 28 days of dental extraction. The presence of inflammation in the BA group can represent toxicity induced by the substance dosage used.

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Funding

This work was supported and funded by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) – [Finance Code 001].

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Authors and Affiliations

  1. School of Health and Life Sciences, Post-Graduate Program in Dentistry, Pontifical Catholic University of Rio Grande do Sul, PUCRS, Avenida Ipiranga, 6681, Partenon, Porto Alegre, RS, 90619-900, Brazil

    Renan R. da Costa, Gabriela da Cunha & João B.B. Weber

  2. School of Medicine, Post-Graduate Program in Medicine and Health Sciences, Pontifical Catholic University of Rio Grande do Sul, PUCRS, Porto Alegre, RS, Brazil

    Raquel D. S. Freitas

  3. Nutrition Thinking® Co., Tecnopuc, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, RS, Brazil

    Raquel D. S. Freitas

Authors
  1. Renan R. da Costa
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  2. Gabriela da Cunha
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  3. Raquel D. S. Freitas
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  4. João B.B. Weber
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Correspondence to João B.B. Weber.

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da Costa, R.R., da Cunha, G., Freitas, R.D.S. et al. Effects of boric acid on alveolar sockets filling after dental extraction in rats. Oral Maxillofac Surg 28, 355–361 (2024). https://doi.org/10.1007/s10006-023-01151-9

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