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Proteomic analysis of human dental pulp in different clinical diagnosis

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Abstract

Objectives

The present study aimed to identify proteins obtained from pulp tissue and correlate with each clinical diagnosis (healthy pulp, inflamed pulp, and necrotic pulp).

Materials and methods

A total of forty-five molars were used. Three biological replicas were evaluated. Lysis and sonication were used for protein extraction. Protein quantification was assessed by using the Bradford technique, and shotgun proteome analysis was performed by nanoUPLC-MSE using a Synapt G2 mass spectrometer. Mass spectra data were processed using the Waters PLGS software, and protein identification was done using the human Uniprot database appended to the PLGS search engine.

Results

A total of 123 different proteins were identified in all evaluated pulp conditions. Among these, 66 proteins were observed for healthy pulp, 66 for inflamed pulp, and 91 for necrotic pulp. Most protein identification was related to immune response, multi-organism process, platelet activation, and stress in inflamed pulp samples compared to healthy pulp. Proteins related to cellular component organization or biogenesis, developmental process, growth, immune response, multi-organism process, response to stimulus, signaling, stress, and transport were identified in cases of apical periodontitis compared to inflamed pulp.

Conclusions

The progression of the disease to inflamed pulp promoted a high abundance of proteins related to the immune system and stress. Comparing the necrotic pulp with inflamed pulp conditions, a high abundance of proteins was noticed related to metabolism, transport, and response between organisms.

Clinical relevance

This finding may assist in future studies of new markers, understanding of tissue engineering, and development of future products.

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Funding

This study was supported by Universidade Católica de Brasília (UCB), Conselho Nacional de Desenvolvimento Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Fundação de Amparo do Distrito Federal (FAPDF) and Fundação de Apoio ao Desenvolvimento do Ensino, Ciência e Tecnologia do Estado de Mato Grosso do Sul (Fundect).

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Authors and Affiliations

  1. Programa de Pós-graduação em Ciências da Saúde, Universidade de Brasília, Brasília, Distrito Federal, Brazil

    Poliana Amanda Oliveira Silva & Taia Maria Berto Rezende

  2. Centro de Análises Proteômicas e Bioquímicas, Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, SGAN 916N – Av. W5 – Campus II – Modulo C, room C-221, Brasília, Distrito Federal, 70.790-160, Brazil

    Stella Maris de Freitas Lima, Mirna de Souza Freire, Octávio Luiz Franco & Taia Maria Berto Rezende

  3. Curso de Odontologia, Universidade Católica de Brasília, Brasília, Distrito Federal, Brazil

    Stella Maris de Freitas Lima & Taia Maria Berto Rezende

  4. Curso de Odontologia, Centro Universitário do Planalto Central Aparecido dos Santos, UNICEPLAC, Brasília, Distrito Federal, Brazil

    Mirna de Souza Freire

  5. Laboratório de Espectrometria de Massa, Embrapa Recursos Genéticos e Biotecnologia, Brasília, Distrito Federal, Brazil

    André Melro Murad

  6. S-Inova Biotech, Pós-graduação em Biotecnologia , Universidade Católica Dom Bosco, Campo Grande, Mato Grosso do Sul, Brazil

    Octávio Luiz Franco

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  1. Poliana Amanda Oliveira Silva
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Silva, P.A.O., Lima, S., Freire, M.d. et al. Proteomic analysis of human dental pulp in different clinical diagnosis. Clin Oral Invest 25, 3285–3295 (2021). https://doi.org/10.1007/s00784-020-03660-3

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