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Selenite and ebselen supplementation attenuates d-galactose-induced oxidative stress and increases expression of SELR and SEP15 in rat lens

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Abstract

Selenite and ebselen supplementation has been shown to possess anti-cataract potential in some experimental animal models of cataract, however, the underlying mechanisms remain unclear. The present study was designed to evaluate the anti-cataract effects and the underlying mechanisms of selenite and ebselen supplementation on galactose induced cataract in rats, a common animal model of sugar cataract. Transmission electron microscopy images of lens fiber cells (LFC) and lens epithelial cells (LEC) were observed in d-galactose-induced experimental cataractous rats treated with or without selenite and ebselen, also redox homeostasis and expression of proteins such as selenoprotein R (SELR), 15kD selenoprotein (SEP15), superoxide dismutase 1 (SOD1), catalase (CAT), β-crystallin protein, aldose reductase (AR) and glucose-regulated protein 78 (GRP78) were estimated in the lenses. The results showed that d-galactose injection injured rat lens and resulted in cataract formation; however, selenite and ebselen supplementation markedly alleviated ultrastructural injury of LFC and LEC. Moreover, selenite and ebselen supplementation could mitigate the oxidative damage in rat lens and increase the protein expressions of SELR, SEP15, SOD1, CAT and β-crystallin, as well as decrease the protein expressions of AR and GRP78. Taken together, these findings for the first time reveal the anti-cataract potential of selenite and ebselen in galactosemic cataract, and provide important new insights into the anti-cataract mechanisms of selenite and ebselen in sugar cataract.

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Abbreviations

AR:

Aldose reductase

CAT:

Catalase

DMSO:

Dimethyl sulfoxide

ECL:

Enhanced chemiluminescence

ER:

Endoplasmic reticulum

GPX:

Glutathione peroxidase

GRP:

Glucose-regulated protein

LEC:

Lens epithelial cell

LFC:

Lens fiber cell

MDA:

Malondialdehyde

MetO:

Methionine sulfoxide

MSR:

Methionine sulfoxide reductase

MTT:

3-(4,5-dimethylthiazol-2-yl)-2,5-diphenylte-trazolium bromide

OLB:

Osmiophilic lamellar bodies

PBS:

Phosphate-buffered saline

PC:

Protein carbonyl

ROS:

Reactive oxygen species

SELR:

Selenoprotein R

SEP15:

15kD selenoprotein

SOD1:

Superoxide dismutase 1

TBS-T:

Tris-buffered saline with Tween

TEM:

Transmission electron microscopy

TSH:

Total mercapto group

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Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (Project No. 21271077). We give our sincere thanks to the faculties from Analytical and Testing Centre of Huazhong University of Science and Technology for their help in the bioanalysis.

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Authors and Affiliations

  1. Hubei Key Laboratory of Bioinorganic Chemistry and Materia Medica, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, 1037 Luoyu Road, Hongshan, Wuhan, Hubei, 430074, People’s Republic of China

    Jie Dai, Jun Zhou, Hongmei Liu & Kaixun Huang

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  1. Jie Dai
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  2. Jun Zhou
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  3. Hongmei Liu
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  4. Kaixun Huang
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Correspondence to Jun Zhou or Kaixun Huang.

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Dai, J., Zhou, J., Liu, H. et al. Selenite and ebselen supplementation attenuates d-galactose-induced oxidative stress and increases expression of SELR and SEP15 in rat lens. J Biol Inorg Chem 21, 1037–1046 (2016). https://doi.org/10.1007/s00775-016-1400-9

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  • DOI: https://doi.org/10.1007/s00775-016-1400-9

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