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Biosynthesis of Salmonella enterica [NiFe]-hydrogenase-5: probing the roles of system-specific accessory proteins

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Abstract

A subset of bacterial [NiFe]-hydrogenases have been shown to be capable of activating dihydrogen-catalysis under aerobic conditions; however, it remains relatively unclear how the assembly and activation of these enzymes is carried out in the presence of air. Acquiring this knowledge is important if a generic method for achieving production of O2-resistant [NiFe]-hydrogenases within heterologous hosts is to be developed. Salmonella enterica serovar Typhimurium synthesizes the [NiFe]-hydrogenase-5 (Hyd-5) enzyme under aerobic conditions. As well as structural genes, the Hyd-5 operon also contains several accessory genes that are predicted to be involved in different stages of biosynthesis of the enzyme. In this work, deletions in the hydF, hydG, and hydH genes have been constructed. The hydF gene encodes a protein related to Ralstonia eutropha HoxO, which is known to interact with the small subunit of a [NiFe]-hydrogenase. HydG is predicted to be a fusion of the R. eutropha HoxQ and HoxR proteins, both of which have been implicated in the biosynthesis of an O2-tolerant hydrogenase, and HydH is a homologue of R. eutropha HoxV, which is a scaffold for [NiFe] cofactor assembly. It is shown here that HydG and HydH play essential roles in Hyd-5 biosynthesis. Hyd-5 can be isolated and characterized from a ΔhydF strain, indicating that HydF may not play the same vital role as the orthologous HoxO. This study, therefore, emphasises differences that can be observed when comparing the function of hydrogenase maturases in different biological systems.

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Abbreviations

Hyd-1:

Hydrogenase-1

Hyd-2:

Hydrogenase-2

Hyd-5:

Hydrogenase-5

MB:

Methylene blue

MBH:

Membrane bound hydrogenase(s)

Rh. leguminosarum :

Rhizobium leguminosarum

R. eutropha :

Ralstonia eutropha

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Acknowledgments

This work was supported in part by EPSRC Grant EP/K031589/1 (AP) and by BBSRC via a Targeted Priority Studentship [Grant Number BB/G016690/1] (FS). We thank David Lloyd for assistance in developing the glove-box methylene blue assay method and Aaron Barnes for conducting preliminary electrochemistry experiments.

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Authors and Affiliations

  1. Division of Molecular Microbiology, School of Life Sciences, University of Dundee, Dundee, DD1 5EH, Scotland, UK

    Lisa Bowman & Frank Sargent

  2. Department of Chemistry, University of York, Heslington, York, YO10 5DD, England, UK

    Jonathan Balbach, Julia Walton & Alison Parkin

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  1. Lisa Bowman
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  2. Jonathan Balbach
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  3. Julia Walton
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  4. Frank Sargent
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  5. Alison Parkin
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Correspondence to Alison Parkin.

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Bowman, L., Balbach, J., Walton, J. et al. Biosynthesis of Salmonella enterica [NiFe]-hydrogenase-5: probing the roles of system-specific accessory proteins. J Biol Inorg Chem 21, 865–873 (2016). https://doi.org/10.1007/s00775-016-1385-4

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  • DOI: https://doi.org/10.1007/s00775-016-1385-4

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