Skip to main content
SpringerLink
Log in

Improvement of the quality of analytical results generated by GC/MS method for characterization of Helichrysum italicum (Roth) G. Don essential oil

  • Research
  • Published:
Accreditation and Quality Assurance Aims and scope Submit manuscript

Abstract

Most of the papers that treat the composition of essential oils for this purpose use GC/MS or GC/MS and GC/FID techniques for the identification and/or quantification of individual compounds. Therewithal, papers usually treat the composition as semiquantitative (percentage composition as the area of the peak of a particular compound in relation to the total area of ​​the peaks on the chromatogram, given as 100 %). At the same time, the quality of the results is not treated in metrological terms. A GC/MS method for the quantification of components present Helichrysum italicum hydro-distilled essential oil samples from Bosnia and Herzegovina with a similar composition was developed and optimized. The stability of the method setup and adequacy of sample storage and manipulation were assessed. Calibration method and assessment of measurement uncertainty were established for neryl acetate, one of the major compounds with known health benefits.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

Data availability

The data used to support the findings of this study are included within the article.

References

  1. Ćavar Zeljković S, Šolić ME, Maksimović M (2015) Volatiles of Helichrysum italicum (Roth) G. Don from Croatia. Nat Prod Res. https://doi.org/10.1080/14786419.2015.1009458

    Article  PubMed  Google Scholar 

  2. Perrini R, Alba V, Ruta C, Morone-Fortunato I, Blanco A, Montemurro C (2009) An evaluation of a new approach to the regeneration of Helichrysum italicum (Roth) G. Don, and the molecular characterization of the variation among sets of differently derived regenerants. Cell Mol Biol Lett. https://doi.org/10.2478/s11658-009-0007-3

    Article  PubMed  PubMed Central  Google Scholar 

  3. Chinou IB, Roussis V, Perdetzoglou D, Loukis A (1996) Chemical and biological studies on two Helichrysum species of Greek origin. Planta Med. https://doi.org/10.1055/s-2006-957914

    Article  PubMed  Google Scholar 

  4. Hellivan PJ (2009) Immortelle’s sustainable resurgence “New research into the chemistry and application of this "everlasting" natural”. Perfumer &flavorist. https://img.perfumerflavorist.com/files/base/allured/all/document/2009/04/pf.PF_34_05_034_06.pdf

  5. Ninčević T, Grdiša M, Šatović Z, Jug-Dujaković M (2019) Helichrysum italicum (Roth) G. Don: taxonomy, biological activity, biochemical and genetic diversity. Ind Crops Prod. https://doi.org/10.1016/j.indcrop.2019.111487

    Article  Google Scholar 

  6. Paolini J, Desjobert JM, Costa J, Bernardini AF, Buti Castellini C, Cioni PL, Flamini G, Morelli I (2006) Composition of essential oils of Helichrysum italicum (Roth) G. Don fil subsp. italicum from Tuscan archipelago islands. Flav Fragr J. https://doi.org/10.1002/ffj.1726

    Article  Google Scholar 

  7. Maksimovic S, Tadic V, Skala D, Zizovic I (2017) Separation of phytochemicals from Helichrysum italicum: an analysis of different isolation techniques and biological activity of prepared extracts. Phytochemistry. https://doi.org/10.1016/j.phytochem.2017.01.001

    Article  PubMed  Google Scholar 

  8. Uniyal A, Tikara SN, Agrawal OP, Sukumaran D, Veer V (2016) Quantitative evaluation of essential oils for the identification of chemical constituents by gas chromatography/mass spectrometry. Archives of Agriculture and Environmental Science. Retrieved from https://journals.aesacademy.org/index.php/aaes/article/view/aaes-01-01-04

  9. Aşkun T, Başer KHC, Tümen G, Kürkçüoğlu M (2010) Characterization of essential oils of some salvia species and their antimycobacterial activities. Turk J Biol. https://doi.org/10.3906/biy-0809-2

    Article  Google Scholar 

  10. Sneddon J, Masuram S, Richert JC (2007) Gas chromatography-mass spectrometry-basic principles, instrumentation and selected applications for detection of organic compounds. Anal Lett. https://doi.org/10.1080/00032710701300648

    Article  Google Scholar 

  11. JCGM 100:2008, Evaluation of measurement data—guide to the expression of uncertainty in measurement. BIPM. https://www.bipm.org/documents/20126/2071204/JCGM_100_2008_E.pdf

  12. Crowder S, Delker C, Forrest E, Martin N (2020) Introduction to statistics in metrology, chapter 10. Springer, Berlin

    Book  Google Scholar 

  13. Morrison FA (2014) Obtaining uncertainty measures on slope and intercept of a least squares fit with Excel’s LINEST, M.T. University, Ed. https://pages.mtu.edu/~fmorriso/cm3215/UncertaintySlopeInterceptOfLeastSquaresFit.pdf

  14. Lemaire G, Olivero M, Rouquet V, Moga A, Pagnon A, Cenizo V, Portes P (2022) Neryl acetate, the major component of Corsican Helichrysum italicum essential oil, mediates its biological activities on skin barrier. PLoS ONE. https://doi.org/10.1101/2022.04.29.490077

    Article  PubMed  PubMed Central  Google Scholar 

  15. Zheljazkov VD, Semerdjieva I, Yankova-Tsvetkova E, Astatkie T, Stanev S, Dincheva I, Kačániová M (2022) Chemical profile and antimicrobial activity of the essential oils of Helichrysum arenarium (L.) Moench. and Helichrysum italicum (Roth.) G. Don. Plants (Basel). https://doi.org/10.3390/plants11070951

    Article  PubMed  Google Scholar 

  16. Mastelić J, Politeo O, Jerković I (2008) Contribution to the analysis of the essential oil of Helichrysum italicum (Roth.) G. Don. determination of ester bonded acids and phenols. Molecules. https://doi.org/10.3390/molecules13040795

    Article  PubMed  PubMed Central  Google Scholar 

  17. Barwick VJ, Ellison SLR (1999) Measurement uncertainty: approaches to the evaluation of uncertainties associated with recovery. Analyst. https://doi.org/10.1039/A901845J

    Article  Google Scholar 

  18. ICH guideline Q2(R2) on validation of analytical procedures (2022) EMA/CHMP/ICH/82072/2006 Committee for medicinal products for human use. https://www.ema.europa.eu/en/documents/scientific-guideline/ich-guideline-q2r2-validation-analytical-procedures-step-2b_en.pdf

Download references

Acknowledgements

This research was funded by and conducted in the laboratories of the Institute of Metrology of Bosnia and Herzegovina.

Author information

Authors and Affiliations

  1. Institute of Metrology of Bosnia and Herzegovina, Sarajevo, Bosnia and Herzegovina

    Katarina Hafner-Vuk, Vladimir Milojević & Milica Krajišnik

  2. International BURCH University, Sarajevo, Bosnia and Herzegovina

    Monia Avdić & Jasmin Kevrić

Authors
  1. Katarina Hafner-Vuk
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Vladimir Milojević
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Monia Avdić
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jasmin Kevrić
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Milica Krajišnik
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

KH-V and VM originally designed the experiments, did calculations and wrote the main manuscript text. MK performed GCMS measurements. MA and JK supervised the findings of this work.

Corresponding author

Correspondence to Katarina Hafner-Vuk.

Ethics declarations

Conflict of interest

The authors declare that they have no conflicts of interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Hafner-Vuk, K., Milojević, V., Avdić, M. et al. Improvement of the quality of analytical results generated by GC/MS method for characterization of Helichrysum italicum (Roth) G. Don essential oil. Accred Qual Assur 28, 261–270 (2023). https://doi.org/10.1007/s00769-023-01553-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00769-023-01553-y

Keywords

Search

Navigation