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Influence of starting zone on the efficiency of micro-thin-layer chromatography with controlled mobile phase flow rate

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Abstract

The micro-thin-layer chromatograms are presented with the controlled mobile phase flow through the adsorbent layer of HPTLC plate. The chromatograms can be developed with an optimal constant linear velocity of the mobile phase front and therefore higher performance of the chromatographic system can be obtained. The relationships of the plate height vs. linear velocity of the mobile phase front at short development distances are investigated and reported. In addition, the contribution of starting spot variance in total peak variance and the usefulness of different procedures of starting spots narrowing has been studied and discussed.

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Funding

This study was funded by the Polish Ministry of Science and Higher Education.

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Authors and Affiliations

  1. Department of Physical Chemistry, Medical University of Lublin, Chodźki 4a, 20-093, Lublin, Poland

    Aneta Hałka-Grysińska, Adrianna Kardasz & Tadeusz Henryk Dzido

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  1. Aneta Hałka-Grysińska
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  2. Adrianna Kardasz
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  3. Tadeusz Henryk Dzido
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Correspondence to Aneta Hałka-Grysińska.

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Hałka-Grysińska, A., Kardasz, A. & Dzido, T.H. Influence of starting zone on the efficiency of micro-thin-layer chromatography with controlled mobile phase flow rate. JPC-J Planar Chromat 35, 243–250 (2022). https://doi.org/10.1007/s00764-022-00184-2

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  • DOI: https://doi.org/10.1007/s00764-022-00184-2

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