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High-performance thin-layer chromatography (HPTLC) method development and validation for the estimation of curcumin and cineole

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Abstract

Curcumin and cineole are phytochemicals which have shown synergistic effects in allergic rhinitis (AR). The aim of the current work was to develop a simple, rapid, and accurate method for the simultaneous estimation of curcumin and cineole using high-performance thin-layer chromatographic (HPTLC) technique. Aluminum plates pre-coated with silica gel 60F254 were used as the stationary phase. The solvent system ratio of n-hexane–ethyl acetate (6:3.5, V/V) produced sharp, symmetrical, and well-defined peaks for curcumin and cineole, with RF values of 0.31 ± 0.02 and 0.71 ± 0.02, respectively. The absorbance mode at 242 nm was used for densitometric analysis. Curcumin’s linear range was observed between 1 and 6 µg/spot (R2 = 0.995), while cineole’s was 10–60 µg/spot (R2 = 0.997). The limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.045 and 0.135 µg/spot, and 0.034 and 0.102 µg/spot for curcumin and cineole, respectively. The developed method was validated as per the International Council for Harmonisation (ICH) guidelines. The developed method was found to be accurate and sensitive and can be used for the simultaneous estimation of curcumin and cineole in formulations.

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Authors and Affiliations

  1. Department of Drug Regulatory Affairs, Bharati Vidyapeeth (Deemed to be University), Poona College of Pharmacy, Erandwane, Pune, Maharashtra, 411038, India

    Monisha Gamre & Preeti Tamane

  2. Department of Quality Assurance Techniques, Bharati Vidyapeeth (Deemed to be University), Poona College of Pharmacy, Erandwane, Pune, Maharashtra, India

    Shyam Khante & Nilesh Desai-Deshmukh

Authors
  1. Monisha Gamre
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  2. Shyam Khante
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  3. Preeti Tamane
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  4. Nilesh Desai-Deshmukh
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Contributions

Shyam Khanate, Preeti Tamane, and Nilesh Deshmukh contributed to conception and design of the study. Material preparation, data collection, and analysis were performed by Monisha Gamre drafted the manuscript and all authors refined the same. All authors read and approved the final manuscript.

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Correspondence to Nilesh Desai-Deshmukh.

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Gamre, M., Khante, S., Tamane, P. et al. High-performance thin-layer chromatography (HPTLC) method development and validation for the estimation of curcumin and cineole. JPC-J Planar Chromat 35, 43–50 (2022). https://doi.org/10.1007/s00764-022-00155-7

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  • DOI: https://doi.org/10.1007/s00764-022-00155-7

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