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High-performance thin-layer chromatography method development and validation for quantification of naringin in different extracts of Citrus sinensis L. and its antioxidant activity

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Abstract

Citrus sinensis (Linn.), also known as sweet orange, is a conventional culinary herb and has been used in different indigenous medicines, especially in Ayurveda owing to its multifaceted pharmacological profile. A simple, specific, accurate and precise high-performance thin-layer chromatography (HPTLC) method for the quantitative determination and validation of naringin in different extracts of C. sinensis was developed for the first time. The mobile phase of glacial acetic acid‒chloroform‒water‒methanol (1:3:0.1:0.5, V/V) was used for attaining good separation. Densitometric determination was carried out at 296 nm. The calibration curves were reckoned to be linear in the range between 400 and 2000 ng per spot. During the analysis, the ethanolic extract of C. sinensis showed higher content of naringin than dimethyl sulphoxide (DMSO), acetone, and dimethylformamide (DMF) extracts. Further, the antioxidant potential of different extracts of C. sinensis were assessed with the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. The developed method of HPTLC was validated for specificity, linearity, accuracy, and precision. The ethanolic extract has unveiled significant antioxidant activity with a percentage inhibition of 70.45%.

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References

  1. Moore GA (2001) Oranges and lemons: clues to the taxonomy of Citrus from molecular markers. Trends Genet 17(9):536–540. https://doi.org/10.1016/s0168-9525(01)02442-8

    Article  CAS  PubMed  Google Scholar 

  2. Etebu E, Nwauzoma AB (2014) A review on sweet orange (Citrus sinensis Osbeck): health, diseases, and management. Am J Res 2:33–70

    Google Scholar 

  3. Milind P, Orange CD (2012) Range of benefits. Int Res J Pharm 3:59–63

    Google Scholar 

  4. Mehmood B, Dar KK, Ali S, Awan UA, Nayyer AQ, Ghous T (2015) Short Communication: in vitro assessment of antioxidant, antibacterial and phytochemical analysis of peel of Citrus sinensis. Pak J Pharm Sci 28(1):231–239

    PubMed  Google Scholar 

  5. Shalaby NMM, Abd-Alla HI, Ahmed HH, Basoudan N (2011) Protective effect of Citrus sinensis and Citrus aurantifolia against osteoporosis and their phytochemical constituents. J Med Plants Res 5:579–588

    CAS  Google Scholar 

  6. Kumar V, Marković T, Emerald M, Dey A (2016) Herbs: composition and dietary importance. Encycl Food Health. https://doi.org/10.1016/b978-0-12-384947-2.00376-7

    Article  Google Scholar 

  7. Lehrner J, Eckersberger C, Walla P, Pötsch G, Deecke L (2000) Ambient odor of orange in a dental office reduces anxiety and improves mood in female patients. Physiol Behav 71(1–2):83–86. https://doi.org/10.1016/s0031-9384(00)00308-5

    Article  CAS  PubMed  Google Scholar 

  8. Singh P, Shukla R, Prakash B, Kumar A, Singh S, Mishra PK (2010) Chemical profile, antifungal, antiaflatoxigenic and antioxidant activity of Citrus maxima Burm. and Citrus sinensis (L.) Osbeck essential oils and their cyclic monoterpene, DL-limonene. Food Chem Toxicol 48(6):1734–1740. https://doi.org/10.1016/j.fct.2010.04.001

    Article  CAS  PubMed  Google Scholar 

  9. Ribeiro NC, da Camara CA, Born FS, de Siqueira HA (2010) Insecticidal activity against Bemisia tabaci biotype B of peel essential oil of Citrus sinensis var. pear and Citrus aurantium cultivated in northeast Brazil. Nat Prod Commun 5(11):1819–1822

    CAS  PubMed  Google Scholar 

  10. Chinedu E, Arome D, Ameh SF, Ameh GE (2014) Evaluation of the anti-proliferative and cytostatic effect of Citrus sinensis (orange) fruit juice. Int J Appl Basic Med Res. https://doi.org/10.4103/2229-516X.140711

    Article  PubMed  PubMed Central  Google Scholar 

  11. Bagavan A, Rahuman AA, Kamaraj C, Kaushik NK, Mohanakrishnan D, Sahal D (2011) Antiplasmodial activity of botanical extracts against Plasmodium falciparum. Parasitol Res 108(5):1099–1109. https://doi.org/10.1007/s00436-010-2151-0,PMID21079994

    Article  PubMed  Google Scholar 

  12. Bhat GP, Surolia N (2001) In vitro antimalarial activity of extracts of three plants used in the traditional medicine of India. Am J Trop Med Hyg 65(4):304–308. https://doi.org/10.4269/ajtmh.2001.65.304

    Article  CAS  PubMed  Google Scholar 

  13. Trovato A, Monforte MT, Barbera R, Rossitto A, Galati EM, Forestieri AM (1996) Effects of fruit juices of Citrus sinensis L. and Citrus limon L. on experimental hypercholesterolemia in the rat. Phytomedicine 2(3):221–227. https://doi.org/10.1016/S0944-7113(96)80046-8

    Article  CAS  PubMed  Google Scholar 

  14. Cardile V, Graziano AC, Venditti A (2015) Clinical evaluation of Moro (Citrus sinensis (L.) Osbeck) orange juice supplementation for the weight management. Nat Prod Res 15:1–5

    Google Scholar 

  15. Asgary S, Keshvari M (2013) Effects of Citrus sinensis juice on blood pressure. ARYA Atheroscler 9(1):98–101

    PubMed  PubMed Central  Google Scholar 

  16. Puglia C, Offerta A, Saija A, Trombetta D, Venera C (2014) Protective effect of red orange extract supplementation against UV-induced skin damages: photoaging and solar lentigines. J Cosmet Dermatol 13(2):151–157. https://doi.org/10.1111/jocd.12083

    Article  PubMed  Google Scholar 

  17. Stange RRJ, Midland SL, Eckert JW, Sims JJ (1993) An antifungal compound produced by grapefruit and Valencia orange after wounding of the peel. J Nat Prod 56(9):1627–1629. https://doi.org/10.1021/np50099a029

    Article  PubMed  Google Scholar 

  18. Trovato A, Monforte MT, Forestieri AM, Pizzimenti F (2000) In vitro anti-mycotic activity of some medicinal plants containing flavonoids. Boll Chim Farm 139(5):225–227

    CAS  PubMed  Google Scholar 

  19. Camacho-Corona Mdel R, Ramírez-Cabrera MA, Santiago OG, Garza-González E, Palacios Ide P, Luna-Herrera J (2008) Activity against drug resistant-tuberculosis strains of plants used in Mexican traditional medicine to treat tuberculosis and other respiratory diseases. Phytother Res 22(1):82–85. https://doi.org/10.1002/ptr.2269

    Article  PubMed  Google Scholar 

  20. Guzeldag G, Kadioglu L, Mercimek A, Matyar F (2014) Preliminary examination of herbal extracts on the inhibition of Helicobacter pylori. Afr J Tradit Complement Altern Med 11(1):93–96

    PubMed  Google Scholar 

  21. Favela-Hernández JM, González-Santiago O, Ramírez-Cabrera MA, Esquivel-Ferriño PC, Camacho-Corona Mdel R (2016) Chemistry and pharmacology of Citrus sinensis. Molecules 21(2):247. https://doi.org/10.3390/molecules21020247

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. Viswanatha GL, Shylaja H, Moolemath Y (2017) The beneficial role of naringin-a citrus bioflavonoid, against oxidative stress-induced neurobehavioral disorders and cognitive dysfunction in rodents: a systematic review and meta-analysis. Biomed Pharmacother 94:909–929. https://doi.org/10.1016/j.biopha.2017.07.072,PMID28810519

    Article  CAS  PubMed  Google Scholar 

  23. Chen R, Qi QL, Wang MT, Li QY (2016) Therapeutic potential of naringin: an overview. Pharm Biol 54(12):3203–3210. https://doi.org/10.1080/13880209.2016.1216131,PMID27564838

    Article  CAS  PubMed  Google Scholar 

  24. Casoni D, Sima IA, Sarbu C (2014) Thin-layer chromatography-an image processing method for the determination of acidic catecholamine metabolites. J Sep Sci 37:2675–2681

    Article  CAS  PubMed  Google Scholar 

  25. Ketmongkhonsit P, Chaichantipyuth C, Palanuvej C, Thitikornpong W, Sukrong S (2015) A validated TLC–image analysis method for detecting and quantifying bioactive phyllanthin in Phyllanthus amarus and commercial herbal drugs. Songklanakarin J Sci Technol 37:319–326

    CAS  Google Scholar 

  26. Stepfanie NS, Sareh K, Gabriel AA, Teo SS, Farahnaz A, Patrick NO (2015) The HPTLC validated method development for the quantification of naringin from the partially purified Labisia Pumila dichloromethane. J Chromatogr Sep Tech 6:271–274

    Google Scholar 

  27. Alam P, Siddiqui NA, Al-Rehaily AJ (2014) Stability-indicating densitometric high-performance thin-layer chromatographic method for the quantitative analysis of biomarker naringin in the leaves and stems of Rumex vesicarius L. JPC-J Planar Chromatogr 27:204–209. https://doi.org/10.1556/JPC.27.2014.3.10

    Article  CAS  Google Scholar 

  28. Adin SN, Gupta I, Ahad A, Aqil M, Mujeeb M (2022) A developed high-performance thin-layer chromatography method for the determination of baicalin in Oroxylum indicum L. and its antioxidant activity. JPC-J Planar Chromatogr. https://doi.org/10.1007/s00764-022-00182-4

    Article  Google Scholar 

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Author notes

  1. Isha Gupta and Syeda Nashvia Adin have contributed equally to this article and should be considered joint first authors.

Authors and Affiliations

  1. Phytomedicine Laboratory, Department of Pharmacognosy & Phytochemistry, School of Pharmaceutical Education & Research, Jamia Hamdard, New Delhi, 110062, India

    Isha Gupta, Syeda Nashvia Adin & Mohd. Mujeeb

  2. Department of Pharmaceutics, School of Pharmaceutical Education & Research, Jamia Hamdard (Deemed University), New Delhi, 110062, India

    Mohd. Aqil

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  1. Isha Gupta
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  2. Syeda Nashvia Adin
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Gupta, I., Adin, S.N., Aqil, M. et al. High-performance thin-layer chromatography method development and validation for quantification of naringin in different extracts of Citrus sinensis L. and its antioxidant activity. JPC-J Planar Chromat 35, 463–471 (2022). https://doi.org/10.1007/s00764-022-00203-2

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