Skip to main content
SpringerLink
Log in

Validated simultaneous HPTLC analysis of scopoletin and gallic acid in the methanolic fraction of Jatropha glandulifera

  • Original Research Paper
  • Published:
JPC – Journal of Planar Chromatography – Modern TLC Aims and scope Submit manuscript

Abstract

A high-performance thin-layer chromatography method for the simultaneous quantitative determination and validation of scopoletin and gallic acid in the methanolic fraction of Jatropha glandulifera L. was developed for the first time. For achieving good separation, the mobile phase of toluene‒ethyl acetate‒glacial acetic acid (7.5:2.5:0.1, V/V) was used. Densitometric determination was carried out at 254 nm for scopoletin and gallic acid, in reflection/absorption mode; the calibration curves were linear in the range of 100‒600 ng per spot. During the analysis, the methanolic fraction of J. glandulifera L. showed the presence of scopoletin (0.08%) and gallic acid (0.04%). The proposed method is simple, precise, specific, and accurate. The obtained data can be used for the routine analysis of reported biomarkers in crude drug and extracts. The simultaneous quantification and method validation of these biomarkers have not yet been reported in J. glandulifera L. which may be utilized for the proper standardization of the plant.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Burkill HM (1994) The useful plants of west tropical Africa, vol 2. Royal Botanic Gardens, Kew

    Google Scholar 

  2. Cowan MM (1999) Plant products as antimicrobial agents. Clin Microbiol Rev 12(4):564–582

    Article  CAS  Google Scholar 

  3. Ballantine JA (1969) The isolation of two esters of the naphthaquinone alcohol, shikonin, from the shrub Jatropha glandulifera. Phytochemistry 8:1587–1590

    Article  CAS  Google Scholar 

  4. Kozhiparambil K, Purushothaman Sundaram C, et al (1979) Jatropholones A and B, new diterpenoids from the roots of Jatropha gossypiifolia (euphorbiaceae)—crystal structure analysis of Jatropholone B. Tetrahedron Lett 11:979–980

    Article  Google Scholar 

  5. Al-Zanbagi NA, Banaja A-EA, Naija JB (2000) Molluscicidal activity of some Saudi Arabian Euphorbiales against the snail Biomphalaria pfeifferi. J Ethnopharmacol 70:119–125

    Article  CAS  Google Scholar 

  6. Joy PP, Thomas J, Mathew S, Skaria BP (2001) Medicinal plants. In: Bose TK, Kabir J, Joy PP (eds) Tropical horticulture, vol 2. Naya Prakash, Calcutta, pp 449–632

    Google Scholar 

  7. Nayak BS, Patel KN (2009) A marvel plant—Jatropha: an appraisal. Int J Pharm Res 1(3):35–39

    Google Scholar 

  8. Kochhar S, Kochhar VK, Singh SP, Katiyar RS, Pushpangadan P (2005) Differential rooting and sprouting behaviour of two Jatropha species and associated physiological and biochemical changes. Curr Sci 89(6):936–939

    Google Scholar 

  9. Bhagat RB, Kulkarni DK (2013) Phytochemical evaluation and in vitro antimicrobial activity of Jatropha glandulifera Roxb. (family Euphorbiaceae). Int J Pharm Life Sci 4(12):3178–3184

    Google Scholar 

  10. Srinivasan D, Nathan S, Suresh T, Perumalsamy L (2001) Antimicrobial activity of certain Indian medicinal plants used in folkloric medicine. J Ethnopharmacol 74:217–220

    Article  CAS  Google Scholar 

  11. Gaikwad RS, Kakde RB, Kulkarni AU, Gaikwad DR, Panchal VH (2012) In vitro antimicrobial activity of crude extracts of Jatropha species. Curr Bot 3(3):9–15

    Google Scholar 

  12. ICH-Q2A (1994) Text on validation of analytical procedures. In: Harmonized tripartite guideline prepared within the international conference on harmonization of technical requirements for the registration of pharmaceuticals for human use, Geneva

  13. ICH-Q2B (1996) Validation of analytical procedures: methodology. In: Harmonized tripartite guideline prepared within the international conference on harmonization of technical requirements for the registration of pharmaceuticals for human use, Geneva

Download references

Acknowledgements

The authors are thankful to Director, CSIR-NBRI, for providing all the facilities to conduct this research work.

Funding

This work was supported by the Department of Science and Technology, India, under INSPIRE Fellowship Program.

Author information

Authors and Affiliations

  1. Pharmacognosy and Ethnopharmacology Division, CSIR-National Botanical Research Institute, Lucknow, India

    Jyotsana Dwivedi & A. K. S. Rawat

  2. Hygia Institute of Pharmaceutical Education and Research, Lucknow, India

    Abhishek Gupta

  3. Department of Pharmacy, Banasthali Vidyapeeth, Banasthali, India

    Jyotsana Dwivedi & Sarvesh Paliwal

Authors
  1. Jyotsana Dwivedi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Abhishek Gupta
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Sarvesh Paliwal
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. K. S. Rawat
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. K. S. Rawat.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Dwivedi, J., Gupta, A., Paliwal, S. et al. Validated simultaneous HPTLC analysis of scopoletin and gallic acid in the methanolic fraction of Jatropha glandulifera. JPC-J Planar Chromat 33, 457–462 (2020). https://doi.org/10.1007/s00764-020-00063-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00764-020-00063-8

Keywords

Search

Navigation