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Effects of ROS and caspase-3-like protein on the growth and aerenchyma formation of Potamogeton perfoliatus stem

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Abstract

Aerenchyma formation plays an important role in the survival of Potamogeton perfoliatus in submerged environment. To understand the regulatory role of reactive oxygen species (ROS) and caspase 3-like protein signaling molecules in aerenchyma formation, we investigated the effects of exogenous NADPH oxidase inhibitor (diphenyleneiodonium chloride, DPI), catalase inhibitor (3-amino-1,2,4-triazole, AT), and caspase-3-like protein inhibitor (AC-DEVD-CHO, DEVD) on morphological and physiological characteristics and aerenchyma formation in P. perfoliatus. The results showed that after DPI treatment, caspase-3-like protein activity decreased, ROS-related enzyme activities increased, and H2O2 content decreased, thereby inhibiting aerenchyma formation. When the concentration of DPI was approximately 1 μmol/L, the inhibitory effect was the most obvious. On the contrary, after the AT treatment, caspase-3-like protein activity increased, ROS-related enzyme activities decreased, and the H2O2 content increased, ultimately promoting aerenchyma formation, and the promotion was the most obvious under treatment with approximately 500 μmol/L AT. After DEVD treatment, the inhibition of vegetative growth caused by DPI or AT treatment was alleviated, significantly reducing caspase-3-like activity and inhibiting aerenchyma development. The results of this study show that ROS has a positive regulatory effect on aerenchyma formation, and caspase-3-like protein is activated to promote ROS-mediated aerenchyma formation. This experiment provides a new theoretical basis for further exploration of the signal transduction effects of ROS and caspase-3-like protein in plant cells and their roles in plant development.

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Abbreviations

CK:

Control

PCD:

Programmed cell death

ROS:

Reactive oxygen species

DPI:

Diphenyleneiodonium chloride

AT:

3-Amino-1,2,4-triazole

DEVD:

AC-DEVD-CHO

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Author notes

  1. Qinmi Xie and Zhongxun Yuan contributed equally to this work.

Authors and Affiliations

  1. Breeding Base for State Key Laboratory of Land Degradation and Ecological Restoration of North-Western China, Ningxia University, Yinchuan, 750000, China

    Qinmi Xie, Hui Hou & Xilu Ni

  2. Key Lab for Restoration and Reconstruction of Degraded Ecosystem in North-Western China (Ministry of Education), School of Ecology and Environment, Ningxia University, Yinchuan, 750000, China

    Qinmi Xie, Hui Hou & Xilu Ni

  3. Key Laboratory of Eco-Environments in Three Gorges Reservoir Region (Ministry of Education), Southwest University, Chongqing, 400715, China

    Zhongxun Yuan

  4. Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation, School of Life Sciences, Southwest University, Chongqing, 400715, China

    Zhongxun Yuan

  5. School of Agriculture, Ningxia University, Yinchuan, 750000, China

    Hongliang Zhao & Hao Chen

  6. Ningxia Helan Mountain Forest Ecosystem Research Station, State Forestry Administration, Yinchuan, 750000, China

    Xilu Ni

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  1. Qinmi Xie
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Xie, Q., Yuan, Z., Hou, H. et al. Effects of ROS and caspase-3-like protein on the growth and aerenchyma formation of Potamogeton perfoliatus stem. Protoplasma 260, 307–325 (2023). https://doi.org/10.1007/s00709-022-01780-z

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