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Transgenic expression of rice OsPHR2 increases phosphorus uptake and yield in wheat

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Abstract

Oryza sativa PHOSPHATE RESPONSE2 (OsPHR2) can promote the uptake and use of phosphorus (P) in rice. We introduced OsPHR2 into the winter wheat (Triticum aestivum L.) variety “Zhengmai0856.” OsPHR2 was integrated into the wheat genome with two copy numbers and could be correctly transcribed and expressed. OsPHR2 was mainly expressed in the leaves at the seedling stage. From the jointing to filling stage, OsPHR2 was mainly expressed in the roots, followed by the leaves, with a low expression level in detected the tassels and stems. The transgenic lines exhibited higher P accumulation at each growth stage and increased P uptake intensity in comparison to the wild type under low P and high P conditions. Analysis of the root characteristics showed that the transgenic expression of OsPHR2 increased the maximum root length, total root length, root-to-shoot ratio, and root volume under the conditions of P deficiency or low P. A field experiment showed that the transgenic lines had a higher grain yield than the wild type under low P and high P conditions. The yield of the transgenic lines increased by 6.29% and 3.73% on average compared with the wild type under low P and high P conditions, respectively. Thus, the transgenic expression of OsPHR2 could increase P uptake and yield in wheat, but the effect was more prominent under low P conditions.

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Funding

This work was supported by the National Natural Science Foundation of China (Grant No. 31701510), the Genetically Modified Organisms Breeding Major Projects of China (Grant No. 2016ZX08002005-004), the Science and Technology project of Henan Province (Grant No. 192102110015), and the Science Technology Foundation for Outstanding Young Scientists of Henan Academy of Agricultural Sciences (Grant No. 2020YQ29).

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Authors and Affiliations

  1. Key Laboratory of Wheat Germplasm Resources Innovation and Improvement in Henan Province, Key Laboratory for Wheat Biology of Henan Province, Institute of Crop Molecular Breeding, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, 450002, People’s Republic of China

    Yan Li, Yuhui Fang, Chaojun Peng, Xia Hua, Yu Zhang, Xueli Qi, Zhengling Li, Yumin Wang, Lin Hu & Weigang Xu

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  1. Yan Li
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  2. Yuhui Fang
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Correspondence to Weigang Xu.

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Fig. SI1

Map of the expression cassette of OsPHR2 and bar. a The map of the expression cassette of OsPHR2. b The map of the expression cassette of bar. (PNG 2960 kb)

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Li, Y., Fang, Y., Peng, C. et al. Transgenic expression of rice OsPHR2 increases phosphorus uptake and yield in wheat. Protoplasma 259, 1271–1282 (2022). https://doi.org/10.1007/s00709-021-01702-5

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