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Role of sodium ion transporters and osmotic adjustments in stress alleviation of Cynodon dactylon under NaCl treatment: a parallel investigation with rice

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Abstract

Comparative analyses of the responses to NaCl in Cynodon dactylon and a sensitive crop species like rice could effectively unravel the salt tolerance mechanism in the former. C. dactylon, a wild perennial chloridoid grass having a wide range of ecological distribution is generally adaptable to varying degrees of salinity stress. The role of salt exclusion mechanism present exclusively in the wild grass was one of the major factors contributing to its tolerance. Salt exclusion was found to be induced at 4 days when the plants were treated with a minimum conc. of 200 mM NaCl. The structural peculiarities of the salt exuding glands were elucidated by the SEM and TEM studies, which clearly revealed the presence of a bicellular salt gland actively functioning under NaCl stress to remove the excess amount of Na+ ion from the mesophyll tissues. Moreover, the intracellular effect of NaCl on the photosynthetic apparatus was found to be lower in C. dactylon in comparison to rice; at the same time, the vacuolization process increased in the former. Accumulation of osmolytes like proline and glycine betaine also increased significantly in C. dactylon with a concurrent check on the H2O2 levels, electrolyte leakage and membrane lipid peroxidation. This accounted for the proper functioning of the Na+ ion transporters in the salt glands and also in the vacuoles for the exudation and loading of excess salts, respectively, to maintain the osmotic balance of the protoplasm. In real-time PCR analyses, CdSOS1 expression was found to increase by 2.5- and 5-fold, respectively, and CdNHX expression increased by 1.5- and 2-fold, respectively, in plants subjected to 100 and 200 mM NaCl treatment for 72 h. Thus, the comparative analyses of the expression pattern of the plasma membrane and tonoplast Na+ ion transporters, SOS1 and NHX in both the plants revealed the significant role of these two ion transporters in conferring salinity tolerance in Cynodon.

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Acknowledgements

The authors are grateful to CSIR, New Delhi (Award No. 09/285(0046)/2008-EMR-I), and UGC, ERO, Kolkata (Minor Research Project No. PSW-80/12-13), for the financial support for carrying out this work. The authors are also grateful to the EM Facility, AIIMS, New Delhi, and USIC, Burdwan University, Burdwan, respectively, for TEM and SEM studies.

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  1. Plant Biochemistry Laboratory, Department of Botany, University of North Bengal, Rajarammohunpur, Siliguri, West Bengal, 734011, India

    Swarnendu Roy & Usha Chakraborty

  2. Molecular and Analytical Biochemistry Laboratory, Department of Botany, University of Gour Banga, Mokdumpur, Malda, West Bengal, 732103, India

    Swarnendu Roy

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Correspondence to Usha Chakraborty.

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Roy, S., Chakraborty, U. Role of sodium ion transporters and osmotic adjustments in stress alleviation of Cynodon dactylon under NaCl treatment: a parallel investigation with rice. Protoplasma 255, 175–191 (2018). https://doi.org/10.1007/s00709-017-1138-4

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