Abstract
A Na+/H+ antiporter gene from Zoysia japonica (ZjNHX1) which is a member of plant NHX-genes family was cloned by reverse-transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) technology. The isolated cDNA is 2,421 bp in length and contains one open-reading frame (ORF) which comprises 540 amino acid residues and shows higher homology with other plant Na+/H+ antiporters. ZjNHX1 could partially complement the salt-sensitive phenotypes of ∆nhx1 and ∆ena1-4∆nhx1 yeast mutants in the presence of NaCl, KCl, and LiCl. The expression of ZjNHX1 in Z. japonica increased after NaCl treatment and this result accords with that of Na+ contents determination under the same treatment. These results implied that ZjNHX1 functions as a vacuolar Na+/H+ antiporter and plays an important role in salt tolerance and ion homeostasis in Z. japonica.
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Abbreviations
- NHX:
-
vacuolar Na+/H+ antiporter
- NHX :
-
vacuolar Na+/H+ antiporter gene
- ORF:
-
open-reading frame
- RACE:
-
rapid amplification of cDNA ends
- C T :
-
threshold cycle values
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Acknowledgments
We thank Prof. Blumwald Eduardo (Department of Pomology, UC Davis) for kindly providing all the yeast strains. We thank Dr. Qin Wang for revising the manuscript. This work was financially supported by National Natural Science Foundation of China.
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Du, Y., Hei, Q., Liu, Y. et al. Isolation and Characterization of a Putative Vacuolar Na+/H+ Antiporter Gene from Zoysia japonica L.. J. Plant Biol. 53, 251–258 (2010). https://doi.org/10.1007/s12374-010-9107-x
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DOI: https://doi.org/10.1007/s12374-010-9107-x