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Three-dimensional ultrastructure of feeding tubes and interconnected endoplasmic reticulum in root-knot nematode-induced giant cells in rose balsam

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Abstract

We investigated the three-dimensional ultrastructure of feeding tubes and the surrounding region in giant cells induced in rose balsam (Impatiens balsamina L.) roots by the root-knot nematode Meloidogyne incognita, using osmium maceration coupled with field emission scanning electron microscopy (FE-SEM). In the roots of 35-day-old galled rose balsam plants, adult nematodes induced the formation of giant cells containing feeding tubes and numerous organelles, including tubular endoplasmic reticulum (ER), cisternal ER, and mitochondria. The feeding tubes were surrounded by fine tubular structures (20–50 nm in diameter), which were in turn surrounded by tubular ER (approximately 120 nm in diameter). The termini of the fine tubular structures appeared to be connected to the surface of the feeding tubes, suggesting that the fine tubular structures were continuous with narrow channels in the feeding tubes. The tubular ER arose from cisternal ER. Large bundles of tubular ER were present near the feeding tube, in the centers of the giant cells, and in the peripheral regions of the giant cells, such as cell wall ingrowths, while smaller bundles of tubular ER formed networks in the giant cells. These observations suggest that tubular ER functions as vascular bundles in giant cells, facilitating the transport of nutrients. We identified capsule-shaped structures (30 μm in diameter) in the giant cells that consisted of smooth, repeatedly branched ER tubules wrapped in several layers of cisternal ER. We propose that lipids and steroids are synthesized at the smooth branched ER and stored in these capsules until needed by the nematode.

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Abbreviations

CWI:

Cell wall ingrowth

FT:

Feeding tube

GC:

Giant cell

HN:

Head of nematode

St:

Stylet

tER:

Tubular ER

Xy:

Xylem

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  1. Bioproduction Science, Ishikawa Prefectural University, 1-308 Suematsu, Nonoichi, Ishikawa, 921-8836, Japan

    Nao Miyashita & Hironori Koga

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  1. Nao Miyashita
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  2. Hironori Koga
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Correspondence to Hironori Koga.

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Miyashita, N., Koga, H. Three-dimensional ultrastructure of feeding tubes and interconnected endoplasmic reticulum in root-knot nematode-induced giant cells in rose balsam. Protoplasma 254, 1941–1951 (2017). https://doi.org/10.1007/s00709-016-1072-x

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