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Cytological cycles and fates in Psidium myrtoides are altered towards new cell metabolism and functionalities by the galling activity of Nothotrioza myrtoidis

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Abstract

The morphogenesis of galls occurs by the redifferentiation of cells that assume new functions in the modified host plant organs. The redifferentiated cells in the galls of Nothotrioza myrtoidis on Psidium myrtoides have low complexity metabolism and are photosynthesis-deficient. These galls were studied in search for evidences of the establishment of new cell cycles and fates and cytological gradients that corroborate their metabolic profile. Young and mature leaves of P. myrtoides and leaf galls induced by N. myrtoidis at different developmental stages were collected along 24 months and analyzed under light and transmission electron microscopy. The leaves of P. myrtoides are long-lasting and did not senesce within the analyzed period, while the galls have a shorter cycle, and senesce within 1 year. A homogenous parenchyma is established by a “standby-redifferentiation” of the chlorophyllous tissues, and sclerenchyma cells redifferentiate from parenchyma cells in the outer cortex of the mature galls. The lack of organelles, the underdeveloped lamellation of chloroplasts, and the occurrence of few plastoglobules are related to the photosynthetic deficiency of the galls. No cytological gradients were observed, but the organelle-rich cells of the vascular and perivascular parenchymas are similar to those of the nutritive tissues of galls induced by other insect taxa. These cells nearest to the feeding sites of N. myrtoidis present higher metabolism and well-developed apparatus for the prevention of oxidative stress. The features herein described corroborate the low metabolic profile of the galls as the cell cycles and fates of P. myrtoides are manipulated for completely new functionalities.

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Acknowledgments

We thank Fundação de Apoio à Pesquisa do estado de Minas Gerais (FAPEMIG–APQ-00901–11), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq—grant number 307007/2012-2), and Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA–Project: “Manejo e biodiversidade de Psylloidea associados ao sistema integração lavoura-pecuária-floresta e à citricultura no Brasil,” number 02.12.01.028.00.00) for the financial support. We also thank Centro de Microscopia of Universidade Federal de Minas Gerais (CM-UFMG) for the analyses in transmission electron microscopy and M.Sc. BG Ferreira for comments on the manuscript.

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  1. Instituto de Ciências Biológicas, Departamento de Botânica, Universidade Federal de Minas Gerais, Avenida Antônio Carlos, 6627, Pampulha, Belo Horizonte, Minas Gerais, 31270-901, Brazil

    R. G. S. Carneiro & R. M. S. Isaias

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  1. R. G. S. Carneiro
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Correspondence to R. M. S. Isaias.

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Carneiro, R.G.S., Isaias, R.M.S. Cytological cycles and fates in Psidium myrtoides are altered towards new cell metabolism and functionalities by the galling activity of Nothotrioza myrtoidis . Protoplasma 252, 637–646 (2015). https://doi.org/10.1007/s00709-014-0709-x

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