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Studying of quantum dot luminescence quenching effect caused by covalent conjugation with protein

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A Correction to this article was published on 03 October 2017

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Abstract

The characterization of quantum dots (QDs) and their conjugates with bovine serum albumin was done using on-a-chip electrophoresis. Under optimal conditions, bioconjugated QDs were successfully separated from free QDs within ≈10 min. Influence of coupling agent on a quenching of QD fluorescence intensity caused by conjugation to a protein was studied. When using two-step procedure combining 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide with N-hydroxysulfosuccinimide, the highest quenching effect was notable. We showed that QDs and capillary electrophoresis on a chip can be applied as a sensitive technique for the detection of biological molecules. The characteristics of this method are simplicity, small sample and reagent requirements, and high efficiency of separation.

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  • 03 October 2017

    The original version of this article unfortunately contained a mistake. Figure 4 was. The corrected Figure 4 is given below.

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Acknowledgements

This work has been supported by the Grant Agency of the Czech Republic under the contract GP 13-20303P and by the project CEITEC 2020 (LQ1601) with financial support from the Ministry of Education, Youth and Sports of the Czech Republic under the National Sustainability Programme II.

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Authors and Affiliations

  1. Central European Institute of Technology, Brno University of Technology, Purkyňova 123, 612 00, Brno, Czech Republic

    Jelena Pejović Simeunović, Jana Pekárková, Ivana Chamradová & Jaromír Hubálek

  2. Department of Microelectronics, Faculty of Electrical Engineering and Communication, Brno University of Technology, Technická 3058/10, 616 00, Brno, Czech Republic

    Jelena Pejović Simeunović, Jana Pekárková, Jaromír Žák & Jaromír Hubálek

Authors
  1. Jelena Pejović Simeunović
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  2. Jana Pekárková
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  3. Jaromír Žák
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  4. Ivana Chamradová
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  5. Jaromír Hubálek
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Corresponding author

Correspondence to Jelena Pejović Simeunović.

Additional information

The original version of this article was revised: Figure 4 in the original version was wrong. We inserted the corrected figure 4.

A correction to this article is available online at https://doi.org/10.1007/s00706-017-2064-z.

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Simeunović, J.P., Pekárková, J., Žák, J. et al. Studying of quantum dot luminescence quenching effect caused by covalent conjugation with protein. Monatsh Chem 148, 1901–1909 (2017). https://doi.org/10.1007/s00706-017-2038-1

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  • DOI: https://doi.org/10.1007/s00706-017-2038-1

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