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A biosensor for the determination of ammonium ion using flow injection amperometric system

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Abstract

An ammonium ion biosensor was constructed using a screen-printed carbon electrode based on alanine dehydrogenase immobilized on core shell Fe3O4@Au nanoparticles which were attached to the electrode surface by an external magnet. The amperometric current response in a flow injection analysis system depending on the oxidation of NADPH was proportional to the amount of ammonium ion consumed during the catalytic reaction of the alanine dehydrogenase enzyme. The designed biosensor was evaluated with a linear response range from 5 to 250 mM with a sensitivity of 0.2 μA mM−1 cm−2, a detection limit of 2.1 mM, and a quantification limit of 6.3 mM. Moreover, the repeatability (ten measurements) and the reproducibility (three sensors) were estimated as 2.6%RSD and 3.0%RSD, respectively. The proposed biosensor was applied successfully to the determination of ammonium ion concentration in concentrated latex samples.

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Acknowledgements

The authors acknowledge scholarship support from the Department of Chemistry, Faculty of Science, Ubon Ratchathani University (Thailand), the Thailand Research Fund (TRF Grant No. RDG 5750100) and ASEAN-European Academic University Network (ASEA-UNINET).

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Science, Ubon Ratchathani University, Ubon Ratchathani, 34190, Thailand

    Preeyanut Butmee, Sarawut Prasertsri & Anchalee Samphao

  2. Department of Physics, Faculty of Science, Ubon Ratchathani University, Ubon Ratchathani, 34190, Thailand

    Saichol Pimmongkol

  3. National Nanotechnology, National Science and Technology Development Agency, Pathum Thani, 12120, Thailand

    Gamolwan Tumcharern

  4. Department of Chemistry, Faculty of Natural and Mathematical Science, University of Prishtina, Prishtina, Republic of Kosovo

    Albana Veseli

  5. Institute of Chemistry, Analytical Chemistry, Karl Franzens University, 8010, Graz, Austria

    Eda Mehmeti & Kurt Kalcher

  6. Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Ubon Ratchathani University, Ubon Ratchathani, 34190, Thailand

    Anchalee Samphao

Authors
  1. Preeyanut Butmee
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  2. Sarawut Prasertsri
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  3. Saichol Pimmongkol
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  4. Gamolwan Tumcharern
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  5. Albana Veseli
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  6. Eda Mehmeti
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  7. Kurt Kalcher
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  8. Anchalee Samphao
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Correspondence to Anchalee Samphao.

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Butmee, P., Prasertsri, S., Pimmongkol, S. et al. A biosensor for the determination of ammonium ion using flow injection amperometric system. Monatsh Chem 148, 635–644 (2017). https://doi.org/10.1007/s00706-016-1871-y

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  • DOI: https://doi.org/10.1007/s00706-016-1871-y

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