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Biological and molecular characterization of a resistance-breaking isolate of citrus tristeza virus from Uruguay and its effects on Poncirus trifoliata growth performance

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Abstract

Resistance-breaking (RB) isolates of citrus tristeza virus (CTV) can replicate and move systemically in Poncirus trifoliata, a rootstock widely used for management of decline caused by CTV and other purposes. In Uruguay, severe CTV isolates are prevalent, and an RB isolate (designated as RB-UY1) was identified. In order to predict the implications of this genotype circulating in citrus crops grafted on trifoliate rootstocks, the aim of this work was to determine the biological and molecular characteristics of this isolate, the efficiency of its transmission by Toxoptera citricida, and its effects on plant growth performance of P. trifoliata. Our results show that RB-UY1 can be classified as a mild isolate, that it is phylogenetically associated with the RB1 group, and that it is efficiently transmitted by T. citrida. They also suggest that the RB-UY1 isolate should not affect the performance of citrus crops grafted on trifoliate rootstocks, although some growth parameters of P. trifoliata seedlings were affected four years after inoculation.

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Data availability

The datasets generated in the current study are available from the corresponding author.

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Acknowledgements

We thank the INIA citrus sanitation team, who contributed and maintained the plant material and CTV isolates. This research was funded by the National Institute of Agricultural Research (INIA), Uruguay (project CT-22), through the National Program of Citrus Research.

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Authors and Affiliations

  1. Instituto Nacional de Investigación Agropecuaria (INIA), Estación Experimental INIA Salto Grande. Camino al Terrible, Salto, CP 50000, Uruguay

    Leticia Rubio, Ana Arruabarrena, Matías Salvo, Ana Bertalmío, Lester Hernández-Rodríguez, Diego Maeso & Fernando Rivas

  2. Laboratorio de Virología Molecular, Centro Universitario Regional Litoral Norte (CENUR Litoral Norte), Universidad de la República, Rivera 1350, Salto, CP 50000, Uruguay

    Matías Castells, María J. Benítez-Galeano & Rodney Colina

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  1. Leticia Rubio
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Correspondence to Leticia Rubio.

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Communicated by Robert H.A. Coutts

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Rubio, L., Arruabarrena, A., Salvo, M. et al. Biological and molecular characterization of a resistance-breaking isolate of citrus tristeza virus from Uruguay and its effects on Poncirus trifoliata growth performance. Arch Virol 168, 123 (2023). https://doi.org/10.1007/s00705-023-05749-y

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