Abstract
High-throughput sequencing was used to analyze Hibiscus rosa-sinensis (family Malvaceae) plants with virus-like symptoms in Hawaii. Bioinformatic and phylogenetic analysis revealed the presence of two tobamoviruses, hibiscus latent Fort Pierce virus (HLFPV) and a new tobamovirus with the proposed name "hibiscus latent Hawaii virus" (HLHV). This is the first report of the complete sequence, genome organization, and phylogenetic characterization of a tobamovirus infecting hibiscus in Hawaii. RT-PCR with virus-specific primers and Sanger sequencing further confirmed the presence of these viruses. Inoculation experiments showed that HLFPV could be mechanically transmitted to Nicotiana benthamiana and N. tabacum, while HLHV could only be mechanically transmitted to N. benthamiana.
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Data availability
The genomic sequences of hibiscus latent Fort Pierce virus (HLFPV) - Hawaii and hibiscus latent Hawaii virus (HLHV) were submitted to the GenBank database (accession numbers ON614664 and ON602041).
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Acknowledgements
Funding was provided by the National Institute of Food and Agriculture (Hatch HAW09025-H[1001478]), USDA Agricultural Research Service (58-5320-4-012), and the United States Agency for International Development (BFS-G-1100002 and EEM-G-0004-00013).
Funding
This research was supported in part by grants from the National Institute of Food and Agriculture (Hatch HAW09025-H[1001478]), USDA Agricultural Research Service (58-5320-4-012), and the United States Agency for International Development (BFS-G-1100002 and EEM-G-0004-00013).
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All authors contributed to the study conception and design. Material preparation, experiments, and data collection were performed by Xupeng Wang. Data analysis was performed by Xupeng Wang and Alejandro Olmedo-Velarde. John Hu supervised the study and acquired the funding. The first draft of the manuscript was written by Xupeng Wang, and all authors commented on subsequent versions of the manuscript. All authors read and approved the final manuscript.
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Wang, X., Larrea-Sarmiento, A., Olmedo-Velarde, A. et al. First detection and complete genome sequence of a new tobamovirus naturally infecting Hibiscus rosa-sinensis in Hawaii. Arch Virol 168, 40 (2023). https://doi.org/10.1007/s00705-022-05634-0
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DOI: https://doi.org/10.1007/s00705-022-05634-0