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A novel picorna-like virus identified in the cotton boll weevil Anthonomus grandis (Coleoptera: Curculionidae)

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Abstract

The cotton boll weevil (CBW; Anthonomus grandis; Coleoptera: Curculionidae) is considered the major insect pest of cotton, causing considerable losses in yield and fiber quality. An increase in the boll weevil population due to increasingly inefficient chemical control measures is of great concernamong cotton producers. The absence of conventional or transgenic cultivars with minimal resistance to CBW has stimulated the search for new molecular and biological tools for efficient control of this insect pest. In this study, we used a metagenomic approach based on RNA deep sequencing to investigate the presence of viruses and coding viral RNA in apparently healthy native adult CBW insects collected from cotton crops in Mato Grosso state, Brazil. Using an Illumina HiSeq 2000 paired-end platform, 138,798 virus-related reads were obtained, and a consensus sequence of a putative new virus, 10,632 nucleotides in length, was assembled. The sequences of the 5’ and 3’ untranslated regions (UTRs) were determined by rapid amplification of cDNA ends (RACE), followed by Nanopore sequencing. The complete genome sequence included a 5’-UTR (1,158 nucleotides), a 3’-UTR (561 nucleotides), and a single ORF of 8,913 nucleotides encoding a large polyprotein. Sequence analysis of the putative polyprotein showed several regions with high sequence similarity to structural and non-structural proteins of viruses of the family Iflaviridae. Pairwise alignments of polyprotein amino acid sequences showed the highest sequence identity (32.13%) to a partial polyprotein sequence of a putative iflavirus (QKN89051.1) found in samples from wild zoo birds in China. Phylogenetic analysis based on full polyprotein sequences of different iflaviruses indicated that this new picorna-like virus is most closely related to iflaviruses found in lepidopteran insects, and it was therefore tentatively named "Anthonomus grandis iflavirus 1" (AgIV-1). This is, to our knowledge, the first complete viral genome sequence found in CBW, and it could provide a basis for further studies about the infectivity and transmission of this virus and its possible association with symptoms or acute disease. AgIV-1 could potentially be used to develop biological or molecular tools, such as a viral vector to carry interfering RNA molecules for CBW control.

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Funding

This research was supported by grants provided by Fundação de Apoio à Pesquisa do Distrito Federal (FAP-DF, grant number 193.001532/2016) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, grant number 305756/2017-6).

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Authors and Affiliations

  1. Laboratory of Baculovirus, Cell Biology Department, University of Brasília (UnB), Campus Universitário Darcy Ribeiro, Brasília, DF, CEP 70910-900, Brazil

    Leonardo Assis da Silva & Bergmann Morais Ribeiro

  2. BIOMOL/BIOTEC Laboratory, Mato Grosso Cotton Institute (IMAmt), Rondonópolis, MT, 78740-970, Brazil

    Marcos Fernando Basso

Authors
  1. Leonardo Assis da Silva
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  2. Marcos Fernando Basso
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  3. Bergmann Morais Ribeiro
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Correspondence to Bergmann Morais Ribeiro.

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Handling Editor: Simona Abba'.

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Accession number: The partial genome sequence was deposited in the GenBank database under accession number OK413669.

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da Silva, L.A., Basso, M.F. & Ribeiro, B.M. A novel picorna-like virus identified in the cotton boll weevil Anthonomus grandis (Coleoptera: Curculionidae). Arch Virol 168, 29 (2023). https://doi.org/10.1007/s00705-022-05672-8

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