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Complete genome sequence of a novel victorivirus infecting cicada flower (Cordyceps chanhua)

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Abstract

Cicada flower, Cordyceps chanhua, is a precious edible and medicinal mushroom with uses in both medicine and food in China. In this study, Cordyceps chanhua strain RCEF5995 was found to be coinfected by a previously characterized alternavirus, Cordyceps chanhua alternavirus 1 (CcAV1), and a novel victorivirus, tentatively named "Cordyceps chanhua victorivirus 1" (CcV1). Molecular characterization of CcV1 showed that its complete genome is 5,232 nucleotides long with a GC content of 57.5%. Sequence analysis indicated that CcV1 contains two overlapping open reading frames (ORFs), ORF1 and ORF2, encoding a putative coat protein (CP) of 742 amino acids (aa) and a putative RNA-dependent RNA polymerase (RdRp) of 836 aa, respectively. The termination codon of the CP ORF overlaps with the initiation codon of the RdRp ORF at the tetranucleotide sequence AUGA. Homolog searches, sequence comparisons, and phylogenetic analysis based on deduced amino acid sequences of RdRp indicated that CcV1 is a new member of the genus Victorivirus, family Totiviridae.

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Funding

This work was supported by the National Natural Science Foundation of China (Grant no. 32172473) and the Anhui Natural Science Foundation (Grant no. 1908085MC56).

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Author notes

  1. Qiuyan Zhu and Najie Shi contributed equally to this study and share first authorship.

Authors and Affiliations

  1. Anhui Provincial Key Laboratory of Microbial Pest Control, Anhui Agricultural University, Hefei, 230036, China

    Qiuyan Zhu, Najie Shi, Yuxiang Zhang, Fan Peng & Bo Huang

  2. School of Plant Protection, Anhui Agricultural University, Hefei, 230036, China

    Guogen Yang

Authors
  1. Qiuyan Zhu
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  2. Najie Shi
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  3. Yuxiang Zhang
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  4. Fan Peng
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  5. Guogen Yang
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  6. Bo Huang
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Corresponding author

Correspondence to Bo Huang.

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Zhu, Q., Shi, N., Zhang, Y. et al. Complete genome sequence of a novel victorivirus infecting cicada flower (Cordyceps chanhua). Arch Virol 168, 4 (2023). https://doi.org/10.1007/s00705-022-05640-2

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  • DOI: https://doi.org/10.1007/s00705-022-05640-2

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