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Complete genome sequences of three newly discovered cacao mild mosaic virus isolates from Theobroma cacao L. in Brazil and Puerto Rico and evidence for recombination

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Abstract

To analyze the DNA virome associated with cacao (Theobroma cacao L.) trees showing virus-like symptoms in Brazil (BR) and Puerto Rico (PR) during 2018-2019, total DNA was isolated from symptomatic leaves and subjected to high-throughput Illumina sequencing. The assembled complete badnaviral genome sequences were verified by PCR amplification, cloning, and DNA sequencing. Based on pairwise distances and phylogenetic analysis, three badnaviral genomes were identified, and these viruses were found to be isolates of the previously described cacao mild mosaic virus (CaMMV). The three genomes were 7,520, 7,524, and 7,514 bp in size for the isolates CaMMV-BR321, CaMMV-BR322, and CaMMV-PR3, respectively. Each genome contained four predicted open reading frames: ORFs 1-3 and ORFY. The CaMMV-PR3 isolate was identified as a probable recombinant, with a CaMMV-BR-like virus as the major parent.

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Acknowledgements

Funding for this research was provided by MARS Inc. through Trust Agreement 6038-21000-023-12-T and by USDA-ARS, Miami under Agreement FAIN 58-6038-6-008.

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Authors and Affiliations

  1. School of Plant Sciences, University of Arizona, Tucson, AZ, 85721, USA

    Roberto Ramos-Sobrinho, Cory Von Keith & Judith K. Brown

  2. Departament of Cellular Biology, University of Brasília, Brasília, DF, 70910-900, Brazil

    Mayra M. M. Ferro & Tatsuya Nagata

  3. USDA-ARS Subtropical Horticultural Research Station, Miami, FL, 33158, USA

    Alina S. Puig & Osman A. Gutierrez

  4. Mars Center for Cocoa Science, Barro Preto, BA, Brazil

    Dahyana Santos Britto

  5. Mars Wrigley, 434 G Street Suite 200, Davis, CA, 95616, USA

    Jean-Philippe Marelli

Authors
  1. Roberto Ramos-Sobrinho
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  2. Mayra M. M. Ferro
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  3. Tatsuya Nagata
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  4. Alina S. Puig
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  5. Cory Von Keith
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  6. Dahyana Santos Britto
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  7. Osman A. Gutierrez
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  8. Jean-Philippe Marelli
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  9. Judith K. Brown
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Corresponding author

Correspondence to Judith K. Brown.

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This study did not use or involve human participants or animals.

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Handling Editor: Robert H.A. Coutts.

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Ramos-Sobrinho, R., Ferro, M.M.M., Nagata, T. et al. Complete genome sequences of three newly discovered cacao mild mosaic virus isolates from Theobroma cacao L. in Brazil and Puerto Rico and evidence for recombination. Arch Virol 166, 2027–2031 (2021). https://doi.org/10.1007/s00705-021-05063-5

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  • DOI: https://doi.org/10.1007/s00705-021-05063-5

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