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Molecular characterization of a novel partitivirus hosted by the false morel mushroom Gyromitra esculenta

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Abstract

Virus populations of uncultivated fungi remain scarcely studied. In the present study, we characterized a new partitivirus isolated from the false morel mushroom Gyromitra esculenta, named “Gyromitra esculenta partitivirus 1” (GePV1). The complete genome of GePV1, whose sequence was determined by combining high-throughput sequencing and RLM-RACE approaches, comprises two dsRNA segments of 1971 bp and 1799 bp, respectively. Each dsRNA genome segment contains a single open reading frame (ORF), encoding a putative RNA-dependent RNA polymerase (RdRp) and a capsid protein (CP), respectively. The sequences of the RdRp and CP exhibited the highest similarity (69.77% and 47.00% identity, respectively) to those of Rosellinia necatrix partitivirus 2 (RnPV2). Phylogenetic analysis based on the CP and RdRp sequences demonstrated that GePV1 clusters within a clade that includes members of the genus Alphapartitivirus, family Partitiviridae. We propose that GePV1 is a new member of the genus Alphapartitivirus. This is the first study reporting on a new partitivirus identified in the false morel mushroom Gyromitra esculenta.

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Acknowledgements

The authors are thankful to Jilber Barutciyan for his assistance in field studies.

Funding

This study was supported by the Ankara University Coordinatorship of Scientific Research Projects with the project number 19H0430004.

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Authors and Affiliations

  1. Faculty of Science Department of Biology, Ankara University, 06100, Tandogan, Ankara, Turkey

    Ergin Sahin & Ilgaz Akata

  2. Evolutionary Genetics Laboratory (eGL), Faculty of Agriculture Department of Fisheries and Aquaculture, Ankara University, 06110, Dışkapı, Ankara, Turkey

    Emre Keskin

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  1. Ergin Sahin
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  2. Emre Keskin
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  3. Ilgaz Akata
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Correspondence to Ergin Sahin.

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Sahin, E., Keskin, E. & Akata, I. Molecular characterization of a novel partitivirus hosted by the false morel mushroom Gyromitra esculenta. Arch Virol 166, 1247–1251 (2021). https://doi.org/10.1007/s00705-021-04978-3

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