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Molecular detection of kobuviruses in livestock in Northern Ireland and the Republic of Ireland

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Abstract

Kobuviruses have been detected in a wide range of mammals including cats, dogs, pigs, cattle, goats, sheep and bats. Kobuviruses have been detected in symptomatic and asymptomatic animals; however, the clinical significance of infection in animals is still unclear. To date, there is no information regarding kobuvirus prevalence in livestock in Ireland. This study reports the first detection of kobuviruses in pigs, bovines and ovines using quantitative PCR. In this study, mesenteric lymph node was collected from cattle (n = 57), pigs (n = 53) and sheep (n = 50) from farms in Northern Ireland and the Republic of Ireland, from animals which had been submitted by private veterinary practitioners from 2009 to 2011 for routine post mortem and clinico-pathological examination. Kobuviruses were detected in 14 cows (24.5%), 5 pigs (9.4%) and 1 sheep (2%). Phylogenetic analysis of Irish kobuviruses from cattle and pigs revealed that the isolates clustered according to their host species. Interestingly, the sheep kobuvirus clustered with bovine kobuviruses detected in this study and other published kobuvirus strains. The data presented in this study contributes to the understanding of the epidemiology of these viruses in animals and to the genetic diversity that these viruses possess.

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Acknowledgements

This work was funded by the Department of Agriculture and Rural Development for Northern Ireland’s Evidence and Innovation Programme, Project P20110035, entitled “Development and application of improved virus discovery systems and assessment of the potential of pen-side detection methods for emerging, endemic or exotic viral infections of farm animals”.

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Authors and Affiliations

  1. Veterinary Sciences Division, Agri-Food and Biosciences Institute, Belfast, BT4 3SD, Northern Ireland, UK

    P. J. Collins, Michael J. McMenamy, Julie McClintock, Paula Lagan-Tregaskis, Lorna McCabe, Simon Doherty, Michael Welsh & John McKillen

  2. Department of Biological Sciences, Cork Institute of Technology, Rossa Avenue, Bishopstown, Cork, Ireland

    Helen O’Shea

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  1. P. J. Collins
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  2. Michael J. McMenamy
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Correspondence to John McKillen.

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All authors declare that there are no financial or other relationships that might lead to a conflict of interest. All authors have seen and approved the manuscript and have contributed significantly to the work.

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Collins, P.J., McMenamy, M.J., McClintock, J. et al. Molecular detection of kobuviruses in livestock in Northern Ireland and the Republic of Ireland. Arch Virol 162, 1275–1279 (2017). https://doi.org/10.1007/s00705-017-3223-6

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  • DOI: https://doi.org/10.1007/s00705-017-3223-6

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