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Avian reovirus σA and σNS proteins activate the phosphatidylinositol 3-kinase-dependent Akt signalling pathway

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Abstract

The present study was conducted to identify avian reovirus (ARV) proteins that can activate the phosphatidylinositol 3-kinase (PI3K)-dependent Akt pathway. Based on ARV protein amino acid sequence analysis, σA, σNS, μA, μB and μNS were identified as putative proteins capable of mediating PI3K/Akt pathway activation. The recombinant plasmids σA-pcAGEN, σNS-pcAGEN, μA-pcAGEN, μB-pcAGEN and μNS-pcAGEN were constructed and used to transfect Vero cells, and the expression levels of the corresponding genes were quantified by immunofluorescence and Western blot analysis. Phosphorylated Akt (P-Akt) levels in the transfected cells were measured by flow cytometry and Western blot analysis. The results showed that the σA, σNS, μA, μB and μNS genes were expressed in Vero cells. σA-expressing and σNS-expressing cells had higher P-Akt levels than negative control cells, pcAGEN-expressing cells and cells designed to express other proteins (i.e., μA, μB and μNS). Pre-treatment with the PI3K inhibitor LY294002 inhibited Akt phosphorylation in σA- and σNS-expressing cells. These results indicate that the σA and σNS proteins can activate the PI3K/Akt pathway.

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Acknowledgments

We thank Dr. Xiuqing Wang (South Dakota State University) for providing the pcAGEN vector.

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Authors and Affiliations

  1. Department of Biotechnology, Guangxi Key Laboratory of Animal Epidemic Etiology and Diagnostics, Guangxi Veterinary Research Institute, 51 Youai North Road, Nanning, 530001, China

    Liji Xie, Zhixun Xie, Li Huang, Qing Fan, Sisi Luo, Jiaoling Huang, Xianwen Deng, Zhiqin Xie, Tingting Zeng, Yanfang Zhang & Sheng Wang

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  1. Liji Xie
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  2. Zhixun Xie
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  3. Li Huang
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  4. Qing Fan
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  5. Sisi Luo
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  6. Jiaoling Huang
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  7. Xianwen Deng
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  8. Zhiqin Xie
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  9. Tingting Zeng
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  10. Yanfang Zhang
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  11. Sheng Wang
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Correspondence to Zhixun Xie.

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Funding

This study was founded by National Natural Science Foundation of China (no. 31160512), Guangxi Science and Technology Bureau (no. 2013GXNSFBA019120) and Guangxi Government Senior Scientist Foundation (no. 2011B020).

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The authors have no conflicts of interest to declare.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Xie, L., Xie, Z., Huang, L. et al. Avian reovirus σA and σNS proteins activate the phosphatidylinositol 3-kinase-dependent Akt signalling pathway. Arch Virol 161, 2243–2248 (2016). https://doi.org/10.1007/s00705-016-2908-6

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  • DOI: https://doi.org/10.1007/s00705-016-2908-6

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