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Photoelectrochemical biosensor based on DNA aptamers and dual nano-semiconductor heterojunctions for accurate and selective sensing of chloramphenicol

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Abstract 

Nanosheets of anatase TiO2 and CdS quantum dots modified with thioglycolic acid (TGA-CdS QDs) were prepared and hierarchically modified on the indium tin oxides (ITO) electrodes. The heterojunction structure is formed to improve the light capture ability and carrier migration, significantly enhancing the sensitivity of photoelectrochemical (PEC) biosensors. Specific DNA sequences labeled with TGA-CdS QDs were placed on the electrodes to prepare a biosensor for the detection of chloramphenicol with ultrahigh selectivity. In addition, the heterojunction structure and the principle of photocurrent signal amplification on the electrode are described in detail. Under the optimal conditions, the photoelectrochemical biosensors showed good reproducibility and stability for chloramphenicol with a linear response in the range 10–10,000 pM and a limit of detection (LOD) of 0.23 pM. Due to the specific recognition of base pairs, the sensor has excellent anti-interference ability in practical applications. An effective method was developed for the accurate detection of antibiotics with far reaching prospects.

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Funding

This work was supported by the National Natural Science Foundation of China (No. 51802273), Sichuan Science and Technology Program (No. 19YYJC2882), Key Project of Southwest Petroleum University Open Experiments (No. 2020KSZ04030), Key Project of Southwest Petroleum University Open Experiments (2021KSZ04034), Ordinary Project of Southwest Petroleum University Open Experiments (No. 2021KSP04018), and Ordinary Project of Southwest Petroleum University Open Experiments (No. 2021KSP04019).

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  1. State Key Laboratory of Oil & Gas Reservoir Geology and Exploitation, Southwest Petroleum University, Chengdu, 610500, People’s Republic of China

    Jun Yao

  2. School of Chemistry and Chemical Engineering, Southwest Petroleum University, Chengdu, 610500, People’s Republic of China

    Jun Yao & Xiang Zeng

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  1. Jun Yao
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Yao, J., Zeng, X. Photoelectrochemical biosensor based on DNA aptamers and dual nano-semiconductor heterojunctions for accurate and selective sensing of chloramphenicol. Microchim Acta 190, 18 (2023). https://doi.org/10.1007/s00604-022-05573-2

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