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Smartphone-assisted ratiometric fluorescence sensing platform for the detection of doxycycline based on BCNO QDs and calcium ion

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Abstract

A novel colorimetric and ratiometric fluorescence sensor has been established based on boron carbon oxynitride quantum dots (BCNO QDs) and Ca2+ for the detection of doxycycline (DOX). BCNO QDs were synthesized by microwave-assisted method with boric acid and ethylenediamine. The fluorescence of BCNO QDs at 425 nm was quenched due to the electrostatic interaction and inner filter effect with doxycycline. Meanwhile, doxycycline was combined with Ca2+ to form a fluorescence complex, which generated a new fluorescence peak at 520 nm. The fluorescence intensity ratio (F520/F425) has a good linear relationship with doxycycline concentration, and the detection limit is 25 nM. Moreover, the fluorescence of the reaction solution showed a concentration-dependent visual color change from blue to green. In order to facilitate further application, a portable fluorescent test paper which is easy to store was prepared. The RGB values of the reaction solution and corresponding test paper were identified by smartphone, and the visual detection of doxycycline was performed by digital image colorimetric analysis. The application of smartphone and fluorescent test paper can effectively shorten the detection time and simplified the operation, providing an effective scheme for quantitative detection of doxycycline in actual samples. Overall, this work provides a method for the detection of doxycycline and shows that the BCNO QDs have great potential application in food safety.

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Acknowledgements

This work was financially supported by National Natural Science Foundation of China (21707030) and Open Project Funding of the State Key Laboratory of Biocatalysis and Enzyme Engineering (SKLBEE2020017).

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Authors and Affiliations

  1. Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062, China

    Fang Liu, Yu He & Gongwu Song

  2. School of Public Health, North China University of Science and Technology, Tangshan, 063210, Hebei, China

    Manman Wang

  3. Department of Clinical Laboratory, North China University of Science and Technology Affiliated Hospital, Tangshan, 063000, Hebei, China

    Junjian Zhao

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  1. Fang Liu
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  2. Manman Wang
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  3. Yu He
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  4. Gongwu Song
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  5. Junjian Zhao
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Correspondence to Yu He.

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Liu, F., Wang, M., He, Y. et al. Smartphone-assisted ratiometric fluorescence sensing platform for the detection of doxycycline based on BCNO QDs and calcium ion. Microchim Acta 189, 113 (2022). https://doi.org/10.1007/s00604-022-05224-6

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  • DOI: https://doi.org/10.1007/s00604-022-05224-6

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