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Fabrication of hydrophilic zwitterionic microspheres via inverse suspension polymerization for the enrichment of N-glycopeptides

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Abstract

A kind of zwitterionic microsphere was prepared via one-step inverse suspension polymerization employing 3-[N,N-dimethyl-[2-(2-methylpropyl-2-enyloxy) ethyl] ammonium] propane-1-sulfonate (MSA) and N,N-methylene bisacrylamide (BIS) as the precursors. The preparation conditions were carefully investigated and optimized by regulating the content of total monomers, ratio of MSA to BIS, ratio of water to oil, and content of stabilizer. The properties of microspheres were characterized by helium ion microscopy (HIM), Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), N2 adsorption/desorption measurement, and water contact angle measurement. The particle size of resulting polydisperse microspheres ranged from 15–25 μm, exhibiting high specific surface area of 138 m2 g−1. Owing to great hydrophilicity, the resulting zwitterionic microspheres could be directly used as hydrophilic interaction chromatography (HILIC) sorbent to enrich glycopeptides from biosamples without any chemical modification. A total of 19 N-glycopeptides was enriched from 10 μg of IgG digest. Besides, up to 383 N-glycopeptides and 224 N-glycosylation sites were unambiguously identified from 2 μL of human serum digest by cLC-MS/MS after enrichment with zwitterionic microspheres, indicating their great enrichment performance to N-glycopeptides. The approach of preparing hydrophilic zwitterionic microspheres contains only one synthesis reaction and is suitable for large-scale preparation.

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Funding

This work was financially supported by the National Natural Science Foundation of China (No. 21675125) to Y. Shen and the National Natural Sciences Foundation of China (No. 21974137), the CAS-Weigao Research & Development Program ([2017]-009), and the Innovation Program of Science and Research from the Dalian Institute of Chemical Physics (DICPI202005) to J. Ou.

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Authors and Affiliations

  1. Key Laboratory of Synthetic and Natural Functional Molecule of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi’an, 710069, China

    Wenrui Wu, Lei Pan, Chenyang Wang, Jingjing Zhang & Yehua Shen

  2. CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China

    Wenrui Wu, Ruizhi Tang, Lei Pan, Chenyang Wang, Shujuan Ma & Junjie Ou

  3. University of Chinese Academy of Sciences, Beijing, 100049, China

    Junjie Ou

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  1. Wenrui Wu
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  2. Ruizhi Tang
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  3. Lei Pan
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  4. Chenyang Wang
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Wu, W., Tang, R., Pan, L. et al. Fabrication of hydrophilic zwitterionic microspheres via inverse suspension polymerization for the enrichment of N-glycopeptides. Microchim Acta 188, 348 (2021). https://doi.org/10.1007/s00604-021-05010-w

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