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A single nucleotide polymorphism electrochemical sensor based on DNA-functionalized Cd-MOFs-74 as cascade signal amplification probes

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Abstract

An ultrasensitive electrochemical sensor has been constructed for the detection of single nucleotide polymorphisms (SNPs) based on DNA-functionalized Cd-MOFs-74 as cascade signal amplification probe under enzyme-free conditions. Interestingly, the introduction of an auxiliary probe did not disturb the detection of SNP targets, but could bind more Cd-MOFs-74 signal elements to enhance the different pulse voltammetry electrochemical signal 2~3 times as compared to sensing system without auxiliary probe, which obviously improves the sensitivity of the proposed sensor. Experimental results taking p53 tumor suppressor gene as SNP model demonstrated that the proposed method can be employed to sensitively and selectively detect target p53 gene fragment with a linear response ranging from 0.01 to 30 pmol/L (detection limit of 6.3 fmol/L) under enzyme-free conditions. Utilizing this strategy, the ultrasensitive SNP electrochemical sensor is a promising tool for the determination  of SNPs in biomedicine.

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Funding

This work was financially supported by the National Natural Science Foundation of China (NSFC, no. 21765026 and 21864026), Scientific Research Foundation Project of Yunnan Provincial Department of Education (no. 2019J0066), and PhD Scientific Research Foundation of Yunnan Normal University (no. 2018ZB001).

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  1. Jia Li Liu and Yu Chan Ma contributed equally to this work.

Authors and Affiliations

  1. College of Chemistry and Chemical Engineering, Yunnan Normal University, Yunnan, Kunming, 650500, People’s Republic of China

    Jia Li Liu, Yu Chan Ma, Tong Yang, Rong Hu &  Yun Hui Yang

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  1. Jia Li Liu
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  2. Yu Chan Ma
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  3. Tong Yang
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Correspondence to Tong Yang, Rong Hu or Yun Hui Yang.

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Liu, J.L., Ma, Y.C., Yang, T. et al. A single nucleotide polymorphism electrochemical sensor based on DNA-functionalized Cd-MOFs-74 as cascade signal amplification probes. Microchim Acta 188, 266 (2021). https://doi.org/10.1007/s00604-021-04924-9

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