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Sensitive detection of Escherichia coli in diverse foodstuffs by electrochemical aptasensor based on 2D porphyrin-based COF

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Abstract

The two-dimensional porphyrin-based covalent organic framework (denoted by Tph-TDC-COF) was used as the sensitive layerto build an aptamer-based electrochemical sensor for the detection of Escherichia coli (E.coli). Tph-TDC-COF produced with 5,10,15,20-tetrakis(4-aminophenyl)-21H, 23H-porphine (Tph) and [2,2′-bithiophene]-2,5′-dicarbaldehyde (TDC) as building blocks exhibited a highly conjugated structure, outstanding conductivity, large specific surface area, and strong bioaffinity towards aptamers. The adoption of Tph-TDC-COF-modified electrode resulted in improved sensing performance and increased anchoring affinity toward the E.coli-targeted aptamer. Under optimal conditions, the Tph-TDC-COF-based electrochemical aptasensor demonstrated an extremely low detection limit of 0.17 CFU mL−1 for E.coli detection within a linear range of 10 to 1 × 108 CFU mL−1, accompanied by good stability, excellent reproducibility and regeneration ability, and wide practical applications. The current electrochemical aptasensing technique has the potential to be extended to detect different foodborne bacteria using specific aptamer, therefore widening the application of COFs in biosensing and food safety fields.

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Funding

This work was supported by the National Natural Science Foundation of China (No. 51903224), the Excellent Youth Science Foundation (202300410494) and the Scientific and Technological Project (No. 222102220086) of Henan Province, China.

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  1. Henan Provincial Key Laboratory of Surface and Interface Science, Zhengzhou University of Light Industry, No. 136, Science Avenue, Zhengzhou, 450001, People’s Republic of China

    Jing Cui, Yu Zhang, Kan Lun, Baiwei Wu, Linghao He, Minghua Wang, Shaoming Fang, Zhihong Zhang & Liming Zhou

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Cui, J., Zhang, Y., Lun, K. et al. Sensitive detection of Escherichia coli in diverse foodstuffs by electrochemical aptasensor based on 2D porphyrin-based COF. Microchim Acta 190, 421 (2023). https://doi.org/10.1007/s00604-023-05978-7

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