Abstract
A voltammetric biosensor for lead(II) (Pb2+) is described that is based on signal amplification by using an ion-dependent split DNAzyme and template-free DNA extension reaction. The Pb2+-dependent split DNAzyme was assembled on gold nanoparticles (Au@Fe3O4), and this nanoprobe then was exposed to Pb2+ which causes the split-off of DNAzymes to release primers containing 3′-OH groups (S1 and S2). The template-free DNA extension reaction triggers the generation of long ssDNA nanotails, which then can bind the free redox probe N,N′-bis(2-(trimethylammonium iodide)propylene)perylene-3,4,9,10-tetracarboxyldiimide (PDA+) via electrostatic adsorption. Hence, the concentration of PDA+ in solution is reduced. Therefore, less free PDA+ can be immobilized on a glassy carbon electrode modified with electrodeposited gold nanoparticles (depAu) to produce an electrochemical signal, typically measured at ∼0.38 V (vs. SCE) for quantitation of Pb2+. The use of a Pb2+-dependent split DNAzyme avoids the usage of a proteinic enzyme. It also increases the sensitivity of the sensor which has a lower detection limit of 30 pM of Pb2+.
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Acknowledgements
This work was supported by the Fundamental Research Funds for the Central Universities (XDJK2019B022, SWU117045), the Chongqing Research Program of Basic Research and Frontier Technology (cstc2018jcyjA0797) and the national key research and development plan of China (2018YFD0800600).
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Zhang, L., Deng, H., Yuan, R. et al. Electrochemical lead(II) biosensor by using an ion-dependent split DNAzyme and a template-free DNA extension reaction for signal amplification. Microchim Acta 186, 709 (2019). https://doi.org/10.1007/s00604-019-3857-z
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DOI: https://doi.org/10.1007/s00604-019-3857-z