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Competitive aptasensor with gold nanoparticle dimers and magnetite nanoparticles for SERS-based determination of thrombin

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Abstract

It is known that the intensity of surface-enhanced Raman scattering (SERS) of monomeric gold nanoparticles (GNPs) is insufficient for ultrasensitive analysis. The authors describe dimeric GNPs for use in a competitive SERS and aptamer based assay for thrombin. The reagent 1,2-bis(4-pyridyl) ethylene serves as both the coupling agent and the Raman reporter on the GNP dimers. In the presence of thrombin, the hybridization of two aptamers, one attached to the GNP dimers, the other to magnetic nanoparticles, is competitively prevented. This method takes advantage of the unique “hot spots” of the GNP dimers to amplify the Raman signal. This results in an ultra-sensitive thrombin assay when compared to assays using GNP monomers. The limit of detection is as low as 1 fM of thrombin. The Raman intensity, best measured at 1612 cm−1, increases linearly in the 1 fM to 10 nM thrombin concentration range. The method was applied to the determinaiton of thrombin in spiked simulated body fluid and human serum.

This method takes advantage of the unique “hot spots” of the gold nanoparticle dimers to amplify the Raman signal. The dimers are linked to the magnetic nanoparticles via an aptamer. The use of both competitive displacement and magnetic separation greatly improves the sensitivity of the thrombin assay.

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Acknowledgements

This research has been supported by National Natural Science Foundation of China (NSFC) (Grant nos. 21273083 and U1732146) and the Project under Scientific and Technological Planning Grant nos. 2014A040401075 by Guangdong Province and 201805010002 by Guangzhou City.

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  1. MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, Guangzhou Key Laboratory of spectral analysis and functional probes, College of Biophotonics, South China Normal University, Guangzhou, 510631, People’s Republic of China

    Ningjing Jiang, Tingfeng Zhu & Yongjun Hu

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  1. Ningjing Jiang
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  2. Tingfeng Zhu
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  3. Yongjun Hu
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Correspondence to Yongjun Hu.

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Jiang, N., Zhu, T. & Hu, Y. Competitive aptasensor with gold nanoparticle dimers and magnetite nanoparticles for SERS-based determination of thrombin. Microchim Acta 186, 747 (2019). https://doi.org/10.1007/s00604-019-3787-9

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