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A nanocomposite consisting of reduced graphene oxide and electropolymerized β-cyclodextrin for voltammetric sensing of levofloxacin

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Abstract

A glassy carbon electrode (GCE) was modified with a nanocomposite prepared from polymerized β-cyclodextrin (β-CD) and reduced graphene oxide (rGO). The modified GCE is shown to enable the voltammetric determination of traces of levofloxacin (LEV) by various electrochemical techniques. Experimental factors affecting the results including the amount of the substrates in preparation of the nanocomposite, accumulation time, the scan rate and pH value of the electrolyte were optimized. The modified GCE, best operated at a working potential of 1.00 V (vs. Ag/AgCl), has two linear response ranges, one for low LEV concentrations (100 pmol L−1 to 100 nmol L−1), and one for higher LEV concentrations (100 nmol L−1 to 100 μmol L−1). The limit of detection and sensitivity are calculated to be 30 pmol L−1 and 467.33 nA μmol L−1 cm−2, respectively. The modified GCE demonstrates a number of advantages such as high sensitivity and selectivity, low LOD, excellent reproducibility, high surface-to-volume ratio, and good electrocatalytic activity towards LEV. The sensor was successfully applied to the determination of LEV in spiked human serum samples.

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Acknowledgments

This work was partially supported by the SMWK Project no. 02010311 (Germany); DAAD ref. no. 91528917; DFG Project HE 394/3-2 and PUT project 03/32/DSMK/0810.

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Authors and Affiliations

  1. Chemical Injuries Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, 1951683759, Iran

    Mohammad Hossein Ghanbari & Mehdi Rahimi-Nasrabadi

  2. Faculty of Pharmacy, Baqiyatallah University of Medical Sciences, Tehran, 6461853090, Iran

    Mohammad Hossein Ghanbari & Mehdi Rahimi-Nasrabadi

  3. Department of Chemistry, University of Ilam, Ilam, 69315-516, Iran

    Faezeh Shahdost-fard

  4. Faculty of Medicine, Ilam University of Medical Sciences, Ilam, 69391-77143, Iran

    Faezeh Shahdost-fard

  5. Pharmaceutical Sciences Research Center, School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, 6734667149, Iran

    Alireza Khoshroo

  6. Center of Excellence in Electrochemistry, University of Tehran, Tehran, 1951683759, Iran

    Mohammad Reza Ganjali

  7. Biosensor Research Centre, Endocrinology & Metabolism Molecular and Cellular Research Institute, Tehran University of Medical Sciences, Tehran, 1951683759, Iran

    Mohammad Reza Ganjali

  8. Institute of Chemical Technology and Engineering, Faculty of Chemical Technology, Poznan University of Technology, 60965, Poznan, Poland

    Marcin Wysokowski, Sonia Żółtowska-Aksamitowska & Teofil Jesionowski

  9. Institute of Chemistry and Technical Electrochemistry, Faculty of Chemical Technology, Poznan University of Technology, 60965, Poznan, Poland

    Tomasz Rębiś

  10. IESEM, TU Bergakademie, 09599, Freiberg, Germany

    Sonia Żółtowska-Aksamitowska, Parvaneh Rahimi, Yvonne Joseph & Hermann Ehrlich

Authors
  1. Mohammad Hossein Ghanbari
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  2. Faezeh Shahdost-fard
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  3. Alireza Khoshroo
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  4. Mehdi Rahimi-Nasrabadi
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  5. Mohammad Reza Ganjali
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  6. Marcin Wysokowski
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  7. Tomasz Rębiś
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  8. Sonia Żółtowska-Aksamitowska
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  9. Teofil Jesionowski
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  10. Parvaneh Rahimi
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  11. Yvonne Joseph
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  12. Hermann Ehrlich
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Corresponding authors

Correspondence to Mehdi Rahimi-Nasrabadi or Hermann Ehrlich.

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Highlights

• A sensor is based on a modified glassy carbon electrode with a nanocomposite of β-cyclodextrin and reduced graphene oxide.

• A clever combination of the substrates in the nanocomposite has some properties such as high surface to volume ratio and good electrocatalytic effect.

• LEV detection was performed in a range of 100 pmol L-1 to 100 μmol L-1 with a LOD of 30 pmol L-1.

• The sensitivity value was estimated to be 467.33 nA μmol L-1 cm-2.

• Minimum substrates and materials were handled for a possible point of care assays.

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Ghanbari, M.H., Shahdost-fard, F., Khoshroo, A. et al. A nanocomposite consisting of reduced graphene oxide and electropolymerized β-cyclodextrin for voltammetric sensing of levofloxacin. Microchim Acta 186, 438 (2019). https://doi.org/10.1007/s00604-019-3530-6

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