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Electrochemical detection of superoxide anions in HeLa cells by using two enzyme-free sensors prepared from ZIF-8-derived carbon nanomaterials

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Abstract

Two kinds of carbon-based nanozymes were constructed from the same precursor of zeolitic imidazolate framework-8 (ZIF-8) for O2•− determination. Hollow carbon cubic nanomaterial (labelled as HCC) was obtained by chemically etching ZIF-8 with tannic acid and a subsequent calcination. A porous carbon cubic nanomaterial (labelled as PCC) was prepared by directly pyrolysis. Then HCC and PCC were immobilized on the surface of screen printed carbon electrodes (SPCE), fabricating HCC and PCC modified electrodes (denoted as HCC/SPCE and PCC/SPCE). HCC/SPCE, best operated at −0.5 V (vs. Ag/AgCl), has a sensitivity of 6.55 × 102 nA μM−1 cm−2 with a detection limit of 207 nM (at S/N = 3) for O2•− sensing. And PCC/SPCE, best operated at −0.4 V (vs. Ag/AgCl), exhibited a superior performance for O2•− detection with a sensitivity of 1.14 × 103 nA μM−1 cm−2 and a low detection limit of 140 nM (at S/N = 3). The two sensors possess excellent reproducibility and stability. They were used to sense O2•− released from HeLa cells.

Illustration of the synthesis of the hollow carbon cubic nanomaterial (HCC) and of the porous carbon cubic nanomaterial (PCC), and the scheme for detection of superoxide anions in HeLa cell.

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Acknowledgements

This research was financially supported by the Science and Technology Commission of Shanghai Municipality (STCSM, No. 16520710800) and the Fundamental Research Funds for the Central Universities (No. 50321101917022).

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Author notes

  1. Yufei Li and Huanhuan Zhang contributed equally to this work.

Authors and Affiliations

  1. Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, People’s Republic of China

    Yufei Li, Huanhuan Zhang, Xuan Cai, Hongli Zhao & Minbo Lan

  2. Casali Center of Applied Chemistry, Institute of Chemistry, The Hebrew University of Jerusalem, 91904, Jerusalem, Israel

    Shlomo Magdassi

  3. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, People’s Republic of China

    Minbo Lan

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  1. Yufei Li
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  2. Huanhuan Zhang
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  3. Xuan Cai
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  4. Hongli Zhao
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  5. Shlomo Magdassi
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Correspondence to Shlomo Magdassi or Minbo Lan.

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Highlights

• A facile method to fabricate two kinds of carbon-based nanozymes for superoxide sensing.

• Two novel non-enzymatic O2•− sensors were established.

• The recommended two sensors can successfully capture the electrochemical signals of O2•− released from Hela cells.

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Li, Y., Zhang, H., Cai, X. et al. Electrochemical detection of superoxide anions in HeLa cells by using two enzyme-free sensors prepared from ZIF-8-derived carbon nanomaterials. Microchim Acta 186, 370 (2019). https://doi.org/10.1007/s00604-019-3473-y

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