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Nanostructured MXene-based biomimetic enzymes for amperometric detection of superoxide anions from HepG2 cells

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Abstract

A novel MXene-based biomimetic enzyme was synthesized using adenosine triphosphate (ATP) as a template to modify a Mn3(PO4)2 nanostructure on Mxene-Ti3C2 nanosheets. The resulting composite was used as an electrode material in an electrochemical sensor for superoxide anion (O2•−). It displays excellent catalytic properties which is attributed to the synergistic effects of the two-dimensional conductive substrate and the Mn3(PO4)2 nanoparticles. The addition of ATP results in the formation of a porous and ordered nanostructure of Mn3(PO4)2. This facilitates the electron transfer between O2•− and electrode. The sensor, best operated at 0.75 V (vs. Ag/AgCl), displays a rapid amperometric response with a detection limit of 0.5 nM and an analytical range that extends from 2.5 nM to 14 μM. Conceivably, it has potential in the detection of O2•− released by living cells.

Nanostructured MXenes were synthesized by in-situ growth of Mn3(PO4)2 on Ti3C2 nanosheets under the induction of adenosine triphosphate (ATP). They display enzyme mimickong properties. A sensor fabricated with the composites can be used for the detection of superoxide anions released by HepG2 cells.

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Acknowledgements

We gratefully acknowledge to the financial support by the China Postdoctoral Science Foundation (2016 M602627), National Natural Science Foundation of China (21505108), Chongqing Postdoctoral Science Special Foundation (Xm2016032), Transformative Project for Excellent Scientific and Technological Achievements in University (KJZH17108), Special Program for Chongqing Social Business and People’s Livelihood Guarantee of Science and Technology (cstc2017shmsA30001) and Science Foundation for Youths of Science and Technology Department of Shaanxi Province (2016JQ2026).

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  1. Jiushang Zheng and Bin Wang contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of Luminescent and Real-Time Analytical Chemistry, Ministry of Education, Faculty of Materials and Energy, Southwest University, Chongqing, People’s Republic of China, 400715

    Jiushang Zheng, Bin Wang, Yanzi Jin, Bo Weng & Jiucun Chen

  2. School of Chemistry and Chemical Engineering, Ankang University, Ankang, 725000, People’s Republic of China

    Yanzi Jin

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  1. Jiushang Zheng
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  2. Bin Wang
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Correspondence to Bin Wang or Jiucun Chen.

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Zheng, J., Wang, B., Jin, Y. et al. Nanostructured MXene-based biomimetic enzymes for amperometric detection of superoxide anions from HepG2 cells. Microchim Acta 186, 95 (2019). https://doi.org/10.1007/s00604-018-3220-9

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