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Fluorometric determination of glucose based on a redox reaction between glucose and aminopropyltriethoxysilane and in-situ formation of blue-green emitting silicon nanodots

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Abstract

A method is described for fluorometric detection of glucose. It is based on the finding that silicon nanodots (SNDs) are formed from glucose and aminopropyltriethoxysilane (APTES) under mild experimental conditions. The SNDs thus formed have an average diameter of ∼2 nm, exhibit good water dispersibility, blue fluorescence (with excitation/emission maxima at 410/475 nm), broad pH tolerance, and are photostable. The assay was applied to the quantification of glucose with high sensitivity, good specificity, and over a wide detection range (from 10 μM to 0.9 mM). It was applied to the determination of glucose in spiked serum samples and gave satisfactory results and recoveries.

Schematic presentation of serum glucose detection based on a redox reaction between glucose and aminopropyltriethoxysilane and in-situ formation of blue-green emitting silicon nanodots.

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Acknowledgements

This work was supported in part by the National Natural Science Foundation of China (21205108), the Foundation for University Key Teacher by Henan Province (2017GGJS007), the Key Scientific Research Project in Universities of Henan Province (19A150048), and the Fundamental Research Funds for the Central Universities from Hunan University.

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Authors and Affiliations

  1. Institute of Chemical Biology and Nanomedicine (ICBN), State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, People’s Republic of China

    Qiyong Cai & Yeru Liu

  2. College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou, 450001, People’s Republic of China

    Hongmin Meng & Zhaohui Li

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  1. Qiyong Cai
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  2. Hongmin Meng
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  3. Yeru Liu
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Correspondence to Qiyong Cai or Zhaohui Li.

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Cai, Q., Meng, H., Liu, Y. et al. Fluorometric determination of glucose based on a redox reaction between glucose and aminopropyltriethoxysilane and in-situ formation of blue-green emitting silicon nanodots. Microchim Acta 186, 78 (2019). https://doi.org/10.1007/s00604-018-3189-4

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