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A fluorometric aptasensor for patulin based on the use of magnetized graphene oxide and DNase I-assisted target recycling amplification

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Abstract

A fluorometric patulin (PAT) assay is presented that is based on the use of magnetic reduced graphene oxide (rGO) and DNase I. The fluorescence of the PAT aptamer labelled with 6-carboxyfluorescein (FAM) is quenched by magnetized reduced graphene oxide (rGO-Fe3O4) due to fluorescence resonance energy transfer (FRET). However, in the presence of PAT, the labelled aptamer is stripped off from rGO-Fe3O4. The rGO-Fe3O4 is then magnetically separated so that the fluorescence of free labelled PAT aptamer is restored. DNase I cannot hydrolyze the aptamer on rGO-Fe3O4, but it can cleave the free aptamer-PAT complex. This will release FAM and PAT which can undergo a number of additional cycles to trigger the cleavage of abundant aptamer. Recycling of DNase I-assisted target therefore leads to a strong amplification of fluorescence and consequently to an assay with low limit of detection. The detection limit for PAT is as low as 0.28 μg L−1 which is about 13 times lower than that without using DNase I. The method offers a new approach towards rapid, sensitive and selective detection based on an aptamer. Conceivably, it has a wide scope in that it may be applied to numerous other analytes if appropriate aptamers are available.

Schematic of a fluorometric assay based on the use of magnetic graphene oxide and DNase I. It was applied to the determination of patulin. DNase I was introduced for recycling amplification. The detection limit is about 13 times lower than that without using DNase I. Figure a contains poor quality of text in image. Otherwise, please provide replacement figure file.Thank you. I will provide the figure file.

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References

  1. Champdoré MD, Bazzicalupo P, Napoli LD, Montesarchio D, Fabios GD, Cocozza I, Parracino A, Rossi M, D’Auria S (2007) A new competitive fluorescence assay for the detection of patulin toxin. Anal Chem 79:751–757

    Article  Google Scholar 

  2. Pennacchio A, Ruggiero G, Staiano M, Piccialli G, Oliviero G, Lewkowicz A, Synak A, Bojarski P, D'Auria S (2014) A surface plasmon resonance based biochip for the detection of patulin toxin. Opt Mat 36:1670–1675

    Article  CAS  Google Scholar 

  3. Funari R, Della VB, Carrieri R, Morra L, Gesuele F, Altucci C, Velotta R (2015) Detection of parathion and patulin by quartz-crystal microbalance functionalized by the photonics immobilization technique. Biosens Bioelectron 67:224–229

    Article  CAS  Google Scholar 

  4. Ellington AD, Szostak JW (1990) In vitro selection of RNA molecules that bind specific ligands. Nature 346:818–822

    Article  CAS  Google Scholar 

  5. Tuerk C, Gold L (1990) Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase. Science 249:505–510

    Article  CAS  Google Scholar 

  6. Bayraç C, Eyidoğan F, Öktem HA (2017) DNA aptamer-based colorimetric detection platform for Salmonella Enteritidis. Biosens Bioelectron 98:22–28

    Article  Google Scholar 

  7. Xia YK, Liu MM, Wang LL, Yan A, He WH, Chen M, Lan JM, Xu JX, Guan LH, Chen JH (2017) A visible and colorimetric aptasensor based on DNA-capped single-walled carbon nanotubes for detection of exosomes. Biosens Bioelectron 92:8–15

    Article  CAS  Google Scholar 

  8. Luo Z, Wang Y, Lu X, Chen J, Wei F, Huang Z, Zhou C, Duan Y (2017) Fluorescent aptasensor for antibiotic detection using magnetic bead composites coated with gold nanoparticles and a nicking enzyme. Anal Chim Acta 984:177–184

    Article  CAS  Google Scholar 

  9. Arvand M, Mirroshandel AA (2017) Highly-sensitive aptasensor based on fluorescence resonance energy transfer between l-cysteine capped ZnS quantum dots and graphene oxide sheets for the determination of edifenphos fungicide. Biosens Bioelectron 96:324–331

    Article  CAS  Google Scholar 

  10. Kuang H, Chen W, Xu DH, Xu LG, Zhu YY, Liu LQ, Chu HQ, Peng CF, Xu XL, Zhu SF (2010) Fabricated aptamer-based electrochemical " signal-off" sensor of ochratoxin a. Biosens Bioelectron 26:710–716

    Article  CAS  Google Scholar 

  11. Tabriz MA, Shamsipur MA (2017) High sensitive visible light-driven photoelectrochemical aptasensor for shrimp allergen tropomyosin detection using graphitic carbon nitride-TiO2 nanocomposite. Biosens Bioelectron 98:113–118

    Article  Google Scholar 

  12. Li Q, Lu Z, Tan X, Wang P, Wu L, Shao K, Yin W, Han H (2017) Ultrasensitive detection of aflatoxin B1 by SERS aptasensor based on exonuclease-assisted recycling amplification. Biosens Bioelectron 97:59–64

    Article  CAS  Google Scholar 

  13. Yang C, Wang Y, Marty JL, Yang X (2011) Aptamer-based colorimetric biosensing of Ochratoxin a using unmodified gold nanoparticles indicator. Biosens Bioelectron 26:2724–2727

    Article  CAS  Google Scholar 

  14. Xie S, Chai Y, Yuan Y, Bai L, Yuan R (2014) Development of an electrochemical method for Ochratoxin A detection based on aptamer and loop-mediated isothermal amplification. Biosens Bioelectron 55:324–329

    Article  CAS  Google Scholar 

  15. Kuang L, Cao SP, Zhang L, Li QH, Liu CZ, Liang RP, Qiu JD (2016) A novel nanosensor composed of aptamer bio-dots and gold nanoparticles for determination of thrombin with multiple signals. Biosens Bioelectron 85:798–806

    Article  CAS  Google Scholar 

  16. Lu CH, Yang HH, Zhu CL, Chen X, Chen GN (2009) A graphene platform for sensing biomolecules. Angew Chem Int Ed 48:4785–4787

    Article  CAS  Google Scholar 

  17. Weng X, Neethirajan S (2016) A microfluidic biosensor using graphene oxide and aptamer-functionalized quantum dots for peanut allergen detection. Biosens Bioelectron 85:649–656

    Article  CAS  Google Scholar 

  18. Guo Z, Ren J, Wang J, Wang E (2011) Single-walled carbon nanotubes based quenching of free FAM-aptamer for selective determination of ochratoxin a. Talanta 85:2517–2521

    Article  CAS  Google Scholar 

  19. Abnous K, Danesh NM, Ramezani M, Alibolandi P, Taghdisi SM (2017) Amperometric aptasensor for ochratoxin a based on the use of a gold electrode modified with aptamer, complementary DNA, SWCNTs and the redox marker methylene blue. Microchim Acta 184:81–90

    Article  CAS  Google Scholar 

  20. Ji XT, Yi BQ, Xu YJ, Zhao YN, Zhong H, Ding CF (2017) A novel fluorescent biosensor for adenosine triphosphate detection based on the polydopamine nanospheres integrating with enzymatic recycling amplification. Talanta 169:8–12

    Article  CAS  Google Scholar 

  21. Liu L, Chang Y, Yu J, Jiang M, Xia N (2017) Two-in-one polydopamine nanospheres for fluorescent determination of beta-amyloid oligomers and inhibition of beta-amyloid aggregation. Sens Actuators B-Chem 251:359–365

    Article  CAS  Google Scholar 

  22. Zhang Y, Zheng B, Zhu CF, Zhang X, Tan CL, Li H, Chen B, Yang J, Chen JZ, Huang Y, Wang LH, Zhang H (2015) Single-layer transition metal Dichalcogenide Nanosheet-based nanosensors for rapid, sensitive, and multiplexed detection of DNA. Adv Mater:27935–27939

  23. Mo L, Li J, Liu Q, Qiu L, Tan W (2016) Nucleic acid-functionalized transition metal nanosheets for biosensing applications. Biosens Bioelectron 89:201–211

    Article  Google Scholar 

  24. Sheng LF, Ren JT, Miao YQ, Wang JH, Wang EK (2011) PVP-coated graphene oxide for selective determination of ochratoxin a via quenching fluorescence of free aptamer. Biosens Bioelectron 26:3494–3499

    Article  CAS  Google Scholar 

  25. Taghdisi SM, Danesh NM, Beheshti HR, Ramezani M, Abnous K (2016) A novel fluorescent aptasensor based on gold and silica nanoparticles for the ultrasensitive detection of ochratoxin a. Nanoscale 8:3439–3446

    Article  CAS  Google Scholar 

  26. Wang B, Chen YF, Wu YY, Weng B, Liu YS, Lu ZS, Li CM, Yu C (2016) Aptamer induced assembly of fluorescent nitrogen-doped carbon dots on gold nanoparticles for sensitive detection of AFB1. Biosens Bioelectron 78:23–30

    Article  CAS  Google Scholar 

  27. Chen W, Fang X, Ye X, Li H, Cao H, Kong J (2017) DNA nanomachine-assisted magnetic bead based target recycling and isothermal amplification for sensitive fluorescence determination of interferon-γ. Microchim Acta 184:4869–4877

    Article  CAS  Google Scholar 

  28. Qiang WB, Wang X, Li W, Chen X, Li H, Xu DK (2015) A fluorescent biosensing platform based on the polydopamine nanospheres intergrating with exonuclease III-assisted target recycling amplification. Biosens Bioelectron 71:143–149

    Article  CAS  Google Scholar 

  29. Wei Y, Zhang J, Wang X, Duan Y (2015) Amplified fluorescent aptasensor through catalytic recycling for highly sensitive detection of ochratoxin a. Biosens Bioelectron 65:16–22

    Article  CAS  Google Scholar 

  30. Wu S, Duan N, Zhang W, Zhao S, Wang Z (2016) Screening and development of DNA aptamers as capture probes for colorimetric detection of patulin. Anal Biochem 508:58–64

    Article  CAS  Google Scholar 

  31. Chang H, Tang L, Wang Y, Jiang J, Li J (2010) Graphene fluorescence resonance energy transfer Aptasensor for the thrombin detection. Anal Chem 82:2341–2346

    Article  CAS  Google Scholar 

  32. Zu F, Yan F, Bai Z, Xu J, Wang Y, Huang Y, Zhou X (2017) The quenching of the fluorescence of carbon dots: a review on mechanisms and applications. Microchim Acta 184:1899–1914

    Article  CAS  Google Scholar 

  33. Yan GP, Wang YH, He XX, Wang KM, Liu JQ, Du YD (2013) A highly sensitive label-free electrochemical aptasensor for interferon-gamma detection based on graphene controlled assembly and nuclease cleavage-assisted target recycling amplification. Biosens Bioelectron 44:57–63

    Article  CAS  Google Scholar 

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Acknowledgements

The authors are grateful for the financial support provided by Fundamental Research Funds for the Central Universities (Project NO.XDJK2017B042 and SWU117005).

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Authors and Affiliations

  1. College of Food Science, Southwest University, Chongqing, 400715, China

    Liang Ma, Ting Guo, Shuli Pan & Yuhao Zhang

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  1. Liang Ma
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  2. Ting Guo
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  3. Shuli Pan
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  4. Yuhao Zhang
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Correspondence to Liang Ma or Ting Guo.

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Ma, L., Guo, T., Pan, S. et al. A fluorometric aptasensor for patulin based on the use of magnetized graphene oxide and DNase I-assisted target recycling amplification. Microchim Acta 185, 487 (2018). https://doi.org/10.1007/s00604-018-3023-z

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  • DOI: https://doi.org/10.1007/s00604-018-3023-z

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