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Gold nanocage-based lateral flow immunoassay for immunoglobulin G

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Abstract

The authors describe a gold nanocage-based lateral flow strip biosensor (LFSB) for low-cost and sensitive detection of IgG. This protein was used as a model analyte to demonstrate the proof-of-concept. The method combines the unique optical properties of gold nanocages (GNCs) with highly efficient chromatographic separation. A sandwich-type of immunoreactions occurs on the GNC-based LFSB which has the attractive features of avoiding multiple incubation, separation, and washing steps. The captured GNCs on the purple test zone and control zone of the biosensor are producing characteristic purple bands, and this enables IgG even to be visually detected. Quantitatation was accomplished by reading the intensities of the bands with a portable strip reader. The LFSB fabrication and assay parameters were optimized. The biosensor displays a linear response in the 0.5 to 50 ng⋅mL−1 IgG concentration range, and it has a 15 min assay time. The detection limit is 0.1 ng⋅mL−1 of IgG, which is 2.5 times lower than that when using a gold nanoparticle-based LFSB. In our perception, this assay has a wide potential for the detection of other proteins and species for which respective antibodies are available.

Gold nanocages have been used as a sensitive tag to prepare lateral flow strip biosensors for sensitive detection of immunoglobulin G.

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Acknowledgements

This research was support by the National Institute of Health, Centers of Biomedical Research Excellent (NIH, COBRE, Grant number: 1P20GM109024-01A1). Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the NIH. Y. Yang acknowledges the National Natural Science Foundation of China (Grant No. 21465026,21165023) and the Program for Changjiang Scholars and Innovative Research Team in University, PCSIRT.

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Authors and Affiliations

  1. College of Chemistry and Chemical Engineering, Yunnan Normal University, Kunming, 650500, People’s Republic of China

    Yunhui Yang

  2. Department of Chemistry and Biochemistry, North Dakota State University, Fargo, North Dakota, 58105, USA

    Yunhui Yang, Mehmet Ozsoz & Guodong Liu

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  1. Yunhui Yang
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  2. Mehmet Ozsoz
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  3. Guodong Liu
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Correspondence to Guodong Liu.

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Yang, Y., Ozsoz, M. & Liu, G. Gold nanocage-based lateral flow immunoassay for immunoglobulin G. Microchim Acta 184, 2023–2029 (2017). https://doi.org/10.1007/s00604-017-2176-5

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